April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Elevated Intracellular Free Calcium in Glaucoma Derived Lamina Cribrosa Cells
B. D. Quill; E. M. McElnea; N. G. Docherty; M. Irnaten; R. C. Barry; A. F. Clark; C. J. O’Brien
Author Affiliations & Notes
  • B. D. Quill
    Department of Ophthalmology, Mater Misericordiae Hospital, Dublin, Ireland
    University College Dublin School of Medicine and Health Sciences, University College Dublin, Dublin, Ireland
  • E. M. McElnea
    Department of Ophthalmology, Mater Misericordiae Hospital, Dublin, Ireland
    University College Dublin School of Medicine and Health Sciences, University College Dublin, Dublin, Ireland
  • N. G. Docherty
    Department of Ophthalmology, Mater Misericordiae Hospital, Dublin, Ireland
    University College Dublin School of Medicine and Health Sciences, University College Dublin, Dublin, Ireland
  • M. Irnaten
    Department of Ophthalmology, Mater Misericordiae Hospital, Dublin, Ireland
    University College Dublin School of Medicine and Health Sciences, University College Dublin, Dublin, Ireland
  • R. C. Barry
    University College Dublin School of Medicine and Health Sciences, University College Dublin, Dublin, Ireland
  • A. F. Clark
    Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, Texas
  • C. J. O’Brien
    Department of Ophthalmology, Mater Misericordiae Hospital, Dublin, Ireland
    University College Dublin School of Medicine and Health Sciences, University College Dublin, Dublin, Ireland
  • Footnotes
    Commercial Relationships  B.D. Quill, None; E.M. McElnea, None; N.G. Docherty, None; M. Irnaten, None; R.C. Barry, None; A.F. Clark, None; C.J. O’Brien, None.
  • Footnotes
    Support  National Glaucoma Research (AHAF)
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 2790. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Elevated Intracellular Free Calcium in Glaucoma Derived Lamina Cribrosa Cells
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      B. D. Quill, E. M. McElnea, N. G. Docherty, M. Irnaten, R. C. Barry, A. F. Clark, C. J. O’Brien; Elevated Intracellular Free Calcium in Glaucoma Derived Lamina Cribrosa Cells. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2790.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Methods: : At confluence, LC cells from ‘normal’ and glaucoma donors were serum starved for 24 hours then exposed to isotonic or hypotonic solution to create hypotonic stretch. Cellular protein was harvested between 1 and 30 minutes to measure phosphorylated PKC- by Western blotting. Intracellular free calcium was measured before and during hyotonic stretch using FURA 2AM spectrofluorescence ratios. In addition, cDNA from both cell types was used to run Taqman low density array analysis of twenty genes involved in calcium homeostasis.

Results: : In glaucomatous LC cells, basal and hypotonic stretch induced intracellular calcium levels are significantly higher (0.71 ± 0.03 v 0.66 ± 0.03, 0.94 ± 0.03 v 0.74 ± 0.02; p < 0.05). Glaucomatous LC cells also showed increased basal and induced levels of activated PKC alpha and significant upregulation of the endoplasmic reticulum Ca2+ pump SERCA3 mRNA(6 fold, p<0.05). In contrast the store-operated channel TRPC1 mRNA was 70% lower in glaucomatous LC cells (-0.7 fold, p<0.05).

Conclusions: : Glaucomatous LC cells have elevated intracellular calcium and increased PKC alpha signalling. Therefore, regulating intracellular Ca2+ levels and associated downstream signalling may represent a potential therapeutic target in glaucoma.

Keywords: lamina cribrosa • second messengers • calcium 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept