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G. Kalesnykas, M. Niittykoski, K. Larsson, K. Kaarniranta, K. Åkerman, H. Uusitalo; Mapping of Calcium Signalling in Rat Retina in an Experimental Model of Glaucoma. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2793.
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To understand the functional alterations occurring in glaucomatous retina, we established a method for labelling the retinal ganglion cells (RGCs) with the calcium indicator Fura-2 in intact, flat-mounted retinas of adult rats.
Pharmacologically evoked responses in laser-induced glaucomatous and control retinas were imaged 2 weeks after the initial laser treatment. Gallyas silver staining of optic nerve sections was also performed for the evaluation of the neurodegenerative changes in the same eyes.
Overall analysis of imaging data revealed that responses evoked by high K+ and ATP were reduced in the cells of laser-treated eyes as compared to controls reflecting poorer cellular conditions rather than change in number of cells expressing functional receptors. Relationships between intraocular pressure (IOP), optic nerve damage and functional characteristics within individual cells of laser-treated eyes indicated that the responses induced by high K+ were negatively associated with the IOP increase whereas the responses induced by ATP were positively associated with the area of neurodegenerative axons in the optic nerves.
Calcium imaging used an indicator of neuronal activity is sensitive tool in combination with pharmacological stimulation to map altered physiological characteristics of RGCs in glaucomatous eye.
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