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X. Liu, W. Yu, J. Qiu, G. Cao, N. Li, M. Yu, L. Chen, N. Yan, I.-H. Pang; Evaluation of Monkey Intraocular Pressure by a Rebound Tonometer. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2868.
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To evaluate the usefulness of a rebound tonometer in measuring intraocular pressure (IOP) in the monkey.
The accuracy of the TonoVetTM rebound tonometer was determined in cannulated eyes of anesthetized rhesus monkeys, where IOP was controlled by adjusting the height of a connected perfusate reservoir. To assess the applicability of the equipment in in vivo studies, the circadian fluctuation of IOP and effect of a known IOP-lowering medication, the Travatan® ophthalmic solution, were evaluated in monkeys in a seated posture under light anesthesia.
IOP readings generated by the TonoVet tonometer correlated very well with the actual pressure in the cannulated monkey eye. The linear correlation had a slope of 0.922 ± 0.014 (mean ± SEM, n = 4), a Y-intercept of 3.04 ± 0.61, and a correlation coefficient r2 = 0.97. Using this method, a trend of diurnal IOP fluctuation of the rhesus monkey was demonstrated. The highest IOP was observed at 15:00 (16.7 ± 0.6 mmHg; mean ± SEM, n = 16), the lowest at 9:00 (15.1 ± 0.7 mmHg). The tonometer was also able to detect the IOP change induced by Travatan. A single topical ocular instillation (30 µL) of Travatan induced a small transient IOP increase (1.1 mmHg versus vehicle control; p = 0.26) at 2 h after treatment, followed by a pressure reduction at 23 h (-2.4 mmHg; p < 0.05). Multiple daily dosing with the drug produced persistent IOP-lowering effect. Three consecutive daily Travatan treatment produced ocular hypotension of -2.0 to -2.2 mmHg (p < 0.05) on the following day.
The rebound tonometer was easy to use and accurately measured IOP in the rhesus monkey eye.
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