April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Comparison of Concentration and Dosage of Intravitreal Recombinant IL-1 Receptor Antagonist in the Treatment of Choroidal Neovascular Membranes in a Rat Model
Author Affiliations & Notes
  • C. K. Pan
    Rocky Mountain Lions Eye Institute, University of Colorado Denver, Aurora, Colorado
  • N. Mandava
    Rocky Mountain Lions Eye Institute, University of Colorado Denver, Aurora, Colorado
  • S. Oliver
    Rocky Mountain Lions Eye Institute, University of Colorado Denver, Aurora, Colorado
  • H. Quiroz-Mercado
    Rocky Mountain Lions Eye Institute, University of Colorado Denver, Aurora, Colorado
  • J. L. Olson
    Rocky Mountain Lions Eye Institute, University of Colorado Denver, Aurora, Colorado
  • Footnotes
    Commercial Relationships  C.K. Pan, None; N. Mandava, None; S. Oliver, None; H. Quiroz-Mercado, None; J.L. Olson, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 2971. doi:
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      C. K. Pan, N. Mandava, S. Oliver, H. Quiroz-Mercado, J. L. Olson; Comparison of Concentration and Dosage of Intravitreal Recombinant IL-1 Receptor Antagonist in the Treatment of Choroidal Neovascular Membranes in a Rat Model. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2971.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To compare the percent reduction from control in the size of experimentally-induced choroidal neovascularization (CNV) in rat eyes treated with intravitreal Interleukin-1 (IL-1) receptor antagonist (anakinra) in differing concentrations versus differing dosage regimens.

Methods: : Laser photocoagulation was used to rupture Bruch’s membrane in both groups. In group 1, the rats were assigned to subgroups of varying concentrations; while in group 2, the rats were assigned to subgroups based on varying frequency of intravitreal anakinra. At day 30, digital angiography was used to measure the CNV area. The percent reduction of CNV area was calculated for each subgroup against its control. Pair-wise comparisons were conducted to assess for statistically significant differences between subgroups. A linear regression was also conducted in each group to assess the impact of total dosage on percent reduction.

Results: : With the exception of the lowest concentration subgroup in group 1, all other treatment subgroups in both groups 1 and 2 showed statistically significant reduction of CNV area versus control (p < 0.001). The linear regression analysis suggested a more sensitive relationship of percent reduction compared to total dosage in group 1 than in group 2. However, this relationship was not demonstrated to be statistically significant in this study.

Conclusions: : IL-1 receptor antagonists effectively reduce the growth of choroidal neovascularization in the animal model. It provides a hopeful pharmacologic alternative in the treatment of exudative macular degeneration.

Keywords: choroid: neovascularization • age-related macular degeneration • vascular endothelial growth factor 
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