April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Assessment of Anti-HSV IgG in Tears and Serum in Patients With Keratitis
Author Affiliations & Notes
  • S. Michee
    CHNO des 15-20, Paris, France
  • R. Gineys
    CHNO des 15-20, Paris, France
  • L. Laroche
    CHNO des 15-20, Paris, France
  • V. Borderie
    CHNO des 15-20, Paris, France
  • P. Goldschmidt
    CHNO des 15-20, Paris, France
  • Footnotes
    Commercial Relationships  S. Michee , None; R. Gineys, None; L. Laroche, None; V. Borderie, None; P. Goldschmidt, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 3101. doi:
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      S. Michee, R. Gineys, L. Laroche, V. Borderie, P. Goldschmidt; Assessment of Anti-HSV IgG in Tears and Serum in Patients With Keratitis. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3101.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the clinical relevance of anti-HSV IgG assessment in serum and tears in patients with keratitis.

Methods: : Results from tear and serum samples processed for anti-HSV IgG identification and measurement in our institution between 2000 and 2008 were retrospectively analyzed. Tear and serum anti-HSV IgG levels were reported to albumin tear and serum concentrations. A ratio filtered/dosed anti-HSV IgG exceeding 4 was considered positive. A ratio less than 2 was considered negative and a ratio between 2 and 4 was considered intermediate. Clinical charts were recorded. Patients were divided in four groups according to anti-HSV results as follows: positive anti-HSV IgG in tears and serum, intermediate anti-HSV IgG in tears and positive in serum, negative anti-HSV IgG in tears and positive in serum, negative anti-HSV IgG in tears and serum.

Results: : Three hundred and sixty-four results were identified among which 35 (9.6%) were positive in tears and serum. This latter result was significantly associated with the presence of a corneal ulcer (p = 0.029), stromal opacities (p = 0.039), stromal edema (p = 0.042), corneal vascularization (p = 0.049), neurotrophic keratitis (p = 0.001), and a clinical response to anti-HSV treatment (p = 0.001).

Conclusions: : Those results suggest that anti-HSV IgG assessment in tears might be useful to identify patients with HSV-related corneal conditions.

Keywords: herpes simplex virus • keratitis 
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