Abstract
Purpose: :
Stromal cell drived factor-1 (SDF-1) is essential for angiogenesis, embryogenesis, and tissue repair through circulating bone marrow-derived stem cells. It was reported that SDF-1 has an important role in ocular ischemic diseases, such as proliferative diabetic retinopathy and retinopathy of prematurity. However, that has been elusive in non-ischemic ocular diseases. In this study, we investigated the vitreous levels of SDF-1 and other inflammatory cytokines in non-ischemic ocular diseases, focused on rhegmatogenous retinal detachment (RRD).
Methods: :
The study involved 78 eyes of 78 cases which received vitrectomy: 49 regmatogenous retinal detachment (RRD), 18 macular hole (MH), and 11 epiretinal membrane (ERM). Vitreous samples were collected before vitrectomy. The amount of SDF-1 levels was measured by enzyme-linked immunosorbant assay (ELISA). Other cytokines, such as interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12, or TNF- were quantified by cytometric bead array.
Results: :
RRD had a mean SDF-1 concentration of 68.7 pg/ml, MH had that of 10.0 pg/ml and ERM had that of 21.2pg/ml. SDF-1 concentraion of RRD was significantly higher than that of ERM (P<0.01, Mann-Whitney test). Significant differences were found neither between RRD and MH, nor between MH and ERM. SDF-1 concentration of RRD was significantly correlated with both duration of disease and extent of detached retina, respectively (P<0.001, P<0.05, Spearman rank correlation test). In addition, the concentration of IL-6 and IL-8 of RRD were higher, which were significantly correlated with the concentration of SDF-1.
Conclusions: :
SDF-1 might have an important role associated with tissue repair and inflammation. It was correlated with other inflammatory cytokines.
Keywords: retinal detachment • cytokines/chemokines • wound healing