April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Study on the Safety of Internal Tamponades by Magnetic Resonance Imaging, Electroretinography and Histology Techniques in Animal Models
Author Affiliations & Notes
  • D. Ma
    Eye Institute,
    The University of Hong Kong, Hong Kong, Hong Kong
  • W. Lui
    Eye Institute,
    The University of Hong Kong, Hong Kong, Hong Kong
  • S.-Y. Li
    Eye Institute,
    The University of Hong Kong, Hong Kong, Hong Kong
  • A. C. Y. Lo
    Eye Institute,
    The University of Hong Kong, Hong Kong, Hong Kong
  • D. Wong
    Eye Institute,
    The University of Hong Kong, Hong Kong, Hong Kong
    St. Paul’s Eye Unit, University of Liverpool, Liverpool, United Kingdom
  • K. C. Chan
    Laboratory of Biomedical Imaging and Signal Processing,
    Department of Electronic and Electrical Engineering, Faculty of Engineering,
    The University of Hong Kong, Hong Kong, Hong Kong
  • E. X. Wu
    Laboratory of Biomedical Imaging and Signal Processing,
    Department of Electronic and Electrical Engineering, Faculty of Engineering,
    The University of Hong Kong, Hong Kong, Hong Kong
  • K. K. W. Li
    Eye Institute,
    Research Centre of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine,
    The University of Hong Kong, Hong Kong, Hong Kong
  • The University of Hong Kong 3T MRI unit
    The University of Hong Kong, Hong Kong, Hong Kong
  • Footnotes
    Commercial Relationships  D. Ma, Fluoron GmbH, F; W. Lui, Fluoron GmbH, F; S.-Y. Li, None; A.C.Y. Lo, None; D. Wong, None; K.C. Chan, None; E.X. Wu, None; K.K.W. Li, None.
  • Footnotes
    Support  University Development Fund and the Seed Funding for Basic Research for The University of Hong Kong
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 3185. doi:
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      D. Ma, W. Lui, S.-Y. Li, A. C. Y. Lo, D. Wong, K. C. Chan, E. X. Wu, K. K. W. Li, The University of Hong Kong 3T MRI unit; Study on the Safety of Internal Tamponades by Magnetic Resonance Imaging, Electroretinography and Histology Techniques in Animal Models. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3185.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Silicone oil is often used as a tamponade agent in surgical treatment of severe rhegmatogenous retinal detachment with proliferative vitreoretinopathy in order to stabilize and provide long-term support for the retina. This study aimed to investigate the longitudinal safety of two kinds of silicone oil: Siluron 2000 (density=0.97) and Densiron 68 (density=1.06) as well as their effects on retinal function using MRI scan, electroretinography (ERG), and histological assessment.

Methods: : Ex-vivo: Pig eye model with MRI imaging is used. Freshly harvested pig eyes were filled with silicone oil during the 3-port pas plana vitrectomy (PPV). Immediately after the procedure, 7T T2W MRI imaging was conducted.In-vivo: rabbit model was used for longitudinal study. 3-month old female New Zealand white rabbits underwent 3-port PPV on the right eye with silicone oils filled. 3T T2W MRI imaging and ERG measurement were performed at 1 week and 3 months after PPV. Flash ERG using Ganzfeld system and contact-lens electrode was performed after dark adaptation for 20 minutes, with the forehead as reference and the contralateral ear as ground. Animals were sacrificed 3 months after PPV and their eyes were enucleated for histology after removal of the silicone oil.. Serial sections were stained with hematoxylin and eosin.

Results: : The MRI scan of pig eyes and post-surgery rabbit eyes showed clear silicone oil bubbles surrounded by an aqueous environment. The a-wave, b-wave and implicit time of ERG signals showed no significant difference between the silicone oil-filled eyes and the control eyes for both Siluron 5000 and Densiron 68. Histology data indicated no significant reduction of retinal ganglion cell number for both silicone oils.

Conclusions: : The high-resolution MRI is proved to be a convenient method to reveal the post-operative therapeutic effect of intraocular tamponade agent on retinal re-attachment. Both ERG and histology data indicated good safety and efficacy of both Siluron 2000 and Densiron 68 in our rabbit model.

Keywords: vitreous substitutes • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • electroretinography: non-clinical 
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