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J. shi, C.-W. Wu, S. Jain, J.-H. Chang, D. T. Azar; Expression of Matrix Metalloproteinase-7 in Murine Optic Nerve Following Transection Injury. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3195.
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To determine the expression of matrix metalloproteinases (MMPs) following murine optic nerve injury.
Meningeal sparing optic nerve transection were performed in WT mice. Mice were sacrificed at week one and two, optic nerves were sectioned and confocal immolocalization was performed for MMP-2, -3, -7 and -14, myelin basic protein (MBP) and glial fibrillary acidic protein (GFAP). Optic nerve regeneration was examined in wild type and MMP-7 knock-out mice using DiI tracing.
Immunostaining revealed that MMP-2, MMP-3, and MMP-14 were localized in the meningeal layers of mouse optic nerve, while MMP-7 was localized within the optic nerve. MMP-7 expression was increased following optic nerve injury. Double labeling indicated that MMP-7 is not expressed in oligodendrocytes, but may be expressed in astrocytes. DiI tracing revealed regeneration of some retinal ganglion cell axons.
MMP-7 expression is increased after optic nerve transection suggesting a role for MMPs in scar formation and/or axonal regeneration following optic nerve injury.
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