April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Non-invasive Assessment of Blood-retinal Barrier Changes by Cirrus HD-OCT
Author Affiliations & Notes
  • R. Bernardes
    Association for Innovation and Biomedical Research on Light and Image (AIBILI), Coimbra, Portugal
    Institute of Biophysics and Biomathematics,
    Institute of Biomedical Research on Light and Image (IBILI), Faculty of Medicine, University of Coimbra, Coimbra, Portugal
  • T. Santos
    Association for Innovation and Biomedical Research on Light and Image (AIBILI), Coimbra, Portugal
  • M. Horne
    Carl Zeiss Meditec, Dublin, California
  • J. Cunha-Vaz
    Association for Innovation and Biomedical Research on Light and Image (AIBILI), Coimbra, Portugal
    Center of Ophthalmology,
    Institute of Biomedical Research on Light and Image (IBILI), Faculty of Medicine, University of Coimbra, Coimbra, Portugal
  • Footnotes
    Commercial Relationships  R. Bernardes, None; T. Santos, None; M. Horne, Carl Zeiss Meditec, E; J. Cunha-Vaz, Carl Zeiss Meditec, C.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 3250. doi:
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    • Get Citation

      R. Bernardes, T. Santos, M. Horne, J. Cunha-Vaz; Non-invasive Assessment of Blood-retinal Barrier Changes by Cirrus HD-OCT. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3250.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To assess blood retinal barrier (BRB) changes non-invasive through structural information provided by Cirrus HD-OCT (Optical Coherence Tomography, Carl Zeiss Meditec, Dublin, CA, USA).

Methods: : Blood-retinal barrier has been assessed by fluorescein angiography and, recently, by the Retinal Leakage Analyzer (RLA) (1), a methodology based on confocal scanning laser ophthalmoscope measurements of fluorescein distribution in the retina and vitreous. The recent launch of the Cirrus HD-OCT allows to assess detailed information on the retina structures. Healthy volunteers and patients with age-related macular degeneration, diabetic retinopathy, cystoid macular edema, and choroidal neo-vascularization underwent RLA and Cirrus HD-OCT exams, which were registered through the respective fundus references. Histograms of OCT reflectivity intensity values collected between the ILM (inner limiting membrane) and RPE (retinal pigment epithelium) were built and compared. From the same eyes, histograms were also built and compared between areas of fluorescein leakage and areas of non-leakage, as identified by the RLA.

Results: : Differences were shown to exist on the histograms of reflectivity intensity values of the Cirrus HD-OCT data between healthy volunteers and patients with different pathologies and within these eyes between areas of leakage and areas of non-leakage of fluorescein.

Conclusions: : These findings suggest that the Cirrus HD-OCT may be used for detecting alterations of the BRB non-invasively.Ref. 1 - Bernardes, R.; Dias, J., Cunha-Vaz, J.; Mapping the Human Blood-Retinal Barrier Function; IEEE Trans. Biomed. Eng., 2005, 52, 106-116.

Clinical Trial: : www.clinicaltrials.gov NCT00797524

Keywords: imaging/image analysis: clinical • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • retina 
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