April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Comparison of Retinal Nerve Fiber Layer Thickness Measurements Using Fourier-Domain and Time-Domain Optical Coherence Tomography
Author Affiliations & Notes
  • F. Di Matteo
    Ophthalmology, Universita' Vita-Salute San Raffaele, Milan, Italy
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy
  • M. Fiori
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy
  • P. Bettin
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy
  • M. Gagliardi
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy
  • S. Bianchi Marzoli
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy
  • L. Pierro
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy
  • R. G. Carassa
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy
  • Footnotes
    Commercial Relationships  F. Di Matteo, None; M. Fiori, None; P. Bettin, None; M. Gagliardi, None; S. Bianchi Marzoli, None; L. Pierro, None; R.G. Carassa, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 3338. doi:
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      F. Di Matteo, M. Fiori, P. Bettin, M. Gagliardi, S. Bianchi Marzoli, L. Pierro, R. G. Carassa; Comparison of Retinal Nerve Fiber Layer Thickness Measurements Using Fourier-Domain and Time-Domain Optical Coherence Tomography. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3338.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To compare Fourier-Domain and Time-Domain Optical Coherence Tomography (OCT) measurements of peripapillary retinal nerve fiber layer (RNFL) thickness.

Methods: : This study involved 41 healthy volunteers. One eye was randomly chosen for study purposes. All subjects underwent a complete ophthalmological evaluation and optic nerve head imaging with Fourier-Domain OCT (OPKO Spectral OCT/SLO, software version 1.76, Ophthalmic Technologies Inc, Toronto, Canada) using the "RNFL" protocol and Time-Domain OCT (Stratus OCT, software version 4.0, Carl Zeiss Meditec Inc, Dublin, CA, USA) using the "Fast RNFL Thickness 3.4" protocol. Mean values were compared by paired t-test. The agreement between Spectral OCT/SLO and Stratus OCT was evaluated by Bland-Altman plots.

Results: : Mean age was 38 ± 8.3 years. Average RNFL thickness with Spectral OCT/SLO (109.17 µm) was significantly higher than Stratus OCT (101.17 µm). Spectral OCT estimates were also higher than Stratus OCT in the superior (129.61 µm vs. 127.12 µm respectively, p=0.14), temporal (75.78 µm vs. 69.56 µm, p<0.001), inferior (134.51 µm vs. 127.93 µm, p<0.001), nasal (96.63 µm vs. 79.76 µm, p<0.001) sectors. The difference between Spectral OCT/SLO and Stratus OCT estimates of the average RNFL thickness was within ± 12 µm in 95% of the patients.

Conclusions: : Spectral OCT/SLO RNFL measurements were moderately higher than Stratus OCT in the inferior, temporal and superior sectors. A more significant overestimation was found in the nasal sector.

Keywords: nerve fiber layer • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • optic disc 
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