April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Sequential Probing for Subtelomeric Arms of Chromosome 6 and Centromeric Chromosome 3 in Choroidal Melanoma Fine Needle Aspiration Biopsies
Author Affiliations & Notes
  • T. A. McCannel
    Ophthalmology, Jules Stein Eye Institute UCLA, Los Angeles, California
  • B. L. Burgess
    Ophthalmology, Jules Stein Eye Institute UCLA, Los Angeles, California
  • N. P. Rao
    Pathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, California
  • B. R. Straatsma
    Ophthalmology, Jules Stein Eye Institute UCLA, Los Angeles, California
  • Footnotes
    Commercial Relationships  T.A. McCannel, None; B.L. Burgess, None; N.P. Rao, None; B.R. Straatsma, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 3373. doi:
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      T. A. McCannel, B. L. Burgess, N. P. Rao, B. R. Straatsma; Sequential Probing for Subtelomeric Arms of Chromosome 6 and Centromeric Chromosome 3 in Choroidal Melanoma Fine Needle Aspiration Biopsies. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3373.

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Abstract

Purpose: : To determine if the prognostic value of monosomy 3 testing in fine needle aspiration biopsies (FNAB) of choroidal melanoma can be improved by the inclusion of fluorescent probes specific for the subtelomeric regions of chromosome 6pq.

Methods: : In patients clinically diagnosed with choroidal melanoma, FNAB was obtained at the time of radioactive Iodine-125 plaque placement. Slide smears of aspirates were sequentially probed for the subtelomeric regions of chromosome 6pq and the centromeric region of chromosome 3 and scored for the number of fluorescent signals in up to 300 nuclei per smear. Results were compared with high density mapping array findings.

Results: : Technique of serial serial probing was feasible for samples. Results included patients with monosomy 3, 6p-gain, monosomy 3 and 6p-gain, monosomy 3 and 6q-loss, and normal signal pattern for both chromosomes. In a case where monosomy 3 and 6p-gain were detected concurrently, the aberrations were found to occur in the same nuclei and did not exist as two separate cell populations. Additionally, positive findings of 6p-gain in the absence of monosomy 3 were determined for at least two tumors.

Conclusions: : The inclusion of chromosome 6 testing allows for a positive finding for the presence of tumor in FISH-probed aspirates in which monosomy 3 is not detected. This provides better prognostic information for tumors with lower likelihood of metastatic outcome. The combined use of FISH probes for the centromeric region of chromosome 3 and the subtelomeric regions of 6pq can identify the homogeneity in tumors that contain hybrid monosomy 3 and 6p-gain signal pattern that microarrays cannot address. Additionally, the use of these combined probes reduces the number of tumor samples that are found to have uninformative FISH results.

Keywords: melanoma • oncology 
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