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K. Abe, H. Mishima, K. Sugioka, Y. Shimomura; Effects of Peptide TCDL on Retinal Pigment Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3428.
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© ARVO (1962-2015); The Authors (2016-present)
During the development of proliferative vitreoretinopathy (PVR), retinal pigment epithelial (RPE) cells have been reported to express -smooth muscle actin (-SMA) and contract the proliferative tissue. We previously reported that tetra peptide TCDL derived from SPARC (osteonectin) inhibited the expression of -SMA by corneal fibroblasts (ARVO 2007). To further determine if peptide TCDL could prevent the development of PVR, we investigated the effects of peptide TCDL on the expression of -SMA and collagen gel contraction by RPE cells in this study.
Human RPE cells (ARPE-19) were embedded in a type 1 collagen gel (3.0x105/well) and cultured. Peptide TCDL (1 mM) and/or TGF- β (5 ng/ml) were added to the medium. Subsequently, the cell shape in the gel and the diameter of the gel were assessed. In addition, the expression of -SMA by RPE cells was estimated by western blot method using specific antibody against -SMA.
RPE cells cultured in the collagen gel appeared to be spindle-shaped and the diameter of the gel gradually decreased in proportion to the culture period. In the presence of peptide TCDL, the cells however kept their spherical shape and the gel contraction was completely abolished. When peptide TCDL was added to the medium after a 24-hour cultivation, the gel contraction was inhibited although the spindle-shaped RPE cells did not change their shape. The addition of TGF- β accelerated the gel contraction regardless of the presence of peptide TCDL. Moreover, the expression of -SMA by RPE cells was decreased by the addition of peptide TCDL.
Peptide TCDL inhibited the expression of -SMA and collagen gel contraction by RPE cells. These results suggested that peptide TCDL might have the potential for hindering the progression of PVR.
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