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S. Groman-Lupa, M. Pedroza-Seres, S. Voorduin, M. Linares, M. C. Jimenez-Martinez; Characterization of NK Cell Surface Markers on a Subset of CD8+ T Cells in Patients With Pars Planitis. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3582.
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© ARVO (1962-2015); The Authors (2016-present)
To determine CD85j, CD94, NKG2D and CD158 expression on CD8+CD57+ T cells in patients with pars planitis.
15 pars planitis patients were included. The expression of CD8, CD57, CD85j, CD94, NKG2, and CD158b were assessed by flow cytometry at basal microenvironment. In a second step CD8+ T cells were isolated by positive immunomagnetic selection and stimulated with/without IL-15.
We observed that CD8+CD57+ T cells were increased in patients with pars planitis. CD85j expression was 3.2 times increased on CD8+CD57+ T cells subsets compared to CD8+CD57- T cells subsets (p=0.009). We also observed a higher frequency of CD94+ on CD8+CD57+ t cells than on CD8+CD57- T cells subsets (p< 0.0001). CD158b expression was 8.1 times more frequent on CD8+CD57+ T cells than on CD8+CD57- T cells (p= 0.001). After stimulation with IL-15 we observed changes in the expression of CD158b on CD8+CD57+ T cells and CD8+CD57-.
CD8+CD57+ T cells were increased in patients with pars planitis as has been reported. CD85j, CD94 and CD158b were preferentially expressed on CD8+CD57+ T cells. IL-15 stimulation apparently down regulates CD158b expression on both CD8+CD57+ T cells and CD8+CD57- T cells. Our findings suggest that chronic inflammatory conditions such as pars planitis may induce the expression of killer cell immunoglobulin-like receptors (KIRs). These receptors have been associated to cellular activation or inhibition functions depending on their associated intracytoplasmic regions. The addition of the proinflammatory cytokine IL-15 found in chronic inflammatory conditions may modify the pattern of the KIR receptors in CD8+ T cells subsets. These properties may be used to design a directed immunotherapy using these cellular surface markers.Acknowledgments: This work was partially supported by a grant from National Council of Science and Technology (CONACYT 71291) and Fundacion Conde de Valenciana.
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