April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Does Administration of Topical Prostaglandins Increase Conjunctival Inflammatory Markers in Uveitics?
Author Affiliations & Notes
  • A. Gurbaxani
    Ophthalmology, Moorfields Eye Hospital, Lonodn, United Kingdom
  • A. Sallam
    Ophthalmology, Moorfields Eye Hospital, Lonodn, United Kingdom
  • G. Galatowicz
    Ophthalmology, Institute of Ophthalmology, Lonodn, United Kingdom
  • S. Lightman
    Ophthalmology, Moorfields Eye Hospital, Lonodn, United Kingdom
  • Footnotes
    Commercial Relationships  A. Gurbaxani, None; A. Sallam, None; G. Galatowicz, None; S. Lightman, None.
  • Footnotes
    Support  Project supported by unrestricted grant from Allergan Inc.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 3584. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      A. Gurbaxani, A. Sallam, G. Galatowicz, S. Lightman; Does Administration of Topical Prostaglandins Increase Conjunctival Inflammatory Markers in Uveitics?. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3584.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To determine whether topical prostaglandins increase the expression of conjunctival inflammatory markers in uveitics and to determine if this is altered by the use of topical steroidsand to extrapolate this information clinicaly into its influence on trabeculectomy blebs in uveitics.

Methods: : A single visit, non-randomized pilot study of 20 patients divided into four groups of 5 patients each (PG only, ST only, PG and ST, no drops). Conjunctival cells harvested by impression cytology and were examined for inflammatory markers (CD3, CD54, HLA-DR, CCR4, CCR5) by flow cytometry. A tear fluid sample was also examined for inflammatory cytokines (IL-12p70, IL-2, IL-10, IL-8, IL-6, IL-4, IL-5, IFN-gamma, IL-1beta, IFN-alpha, IFN-beta).

Results: : No statistical difference was found between any of the groups. All groups demonstrated markers of conjunctival inflammation. CD3, CD54, HLA-DR, CCR4, CCR5, IL-12p70, IL-10, IL-6, IL-4, IFN-gamma, IL-1beta, IFN-alpha, were normally distributed and analysis of variance (ANOVA) did not show any statistical difference between groups. IL-2, IL-8, IL-5 and IFN-beta were not normally distributed, were analyzed using Kruskal Wallis Test and did not show any statistical difference between groups either.

Conclusions: : All uveitis patients examined whether on or off prostaglandin drops had increased inflammatory markers on their conjunctival cells. We can conclude from this pilot study that topical prostaglandins do not increase conjunctival inflammation and this is not modified by topical steroids. Treatment of raised intraocular pressure with topical prostaglandins is therefore not likely to predispose to trabeculectomy failure should trabeculectomy become necessary in the future.

Keywords: uveitis-clinical/animal model • conjunctiva • inflammation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×