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B. Gesslein, H. Morén, S. Andreasson, M. Malmsjö; Multifocal Electroretinogram for Functional Evaluation of Retinal Injury Following Ischemia-Reperfusion in Pigs. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3604.
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© ARVO (1962-2015); The Authors (2016-present)
There is yet no successful pharmacological approach for the treatment of retinal ischemia. Hitherto, research has been performed using small animals and rodents. We have developed a porcine model for retinal ischemia which hopefully will facilitate extrapolation of results to the clinical situation. The aim of the present study was to establish the technique of multifocal electroretinogram (mfERG) in pigs for functional evaluation of retinal injury following ischemia reperfusion
Seven 70 kg pigs underwent pressure-induced retinal ischemia (one hour) followed by reperfusion. mfERG recordings, with simultaneous fundus monitoring, were obtained before and after ischemia followed by one and five hours of reperfusion. Individual components of the summed mfERG responses were correlated to ischemia and the time of reperfusion.
The localized cone function in the central part of the porcine retina could be monitored using mfERG. The visual streak area had significantly higher amplitudes than the optic nerve head area and the inferior retina. The mfERG responses were altered following ischemia-reperfusion. In one group of animals, there was a complete flattening of the mfERG waveforms, indicating complete ischemic injury. In the other group of animals, ischemia reperfusion altered the mfERG such as the implicit time was increased (20.91 ± 0.20 before ischemia and 21.67 ± 0.31 after ischemia and one hour of reperfusion, in the up-regions, p= 0.0492) and the amplitude was decreased (13.18 ± 2.45 before ischemia and 11.32 ±1.35 after ischemia and one hour of reperfusion, in the up-regions, p= 0.5897), suggesting partial ischemic injury.
Previous not shown, the porcine model of pressure-induced retinal ischemia-reperfusion results in mfERG changes similar to that seen in patients with ischemic conditions following e.g. central retinal vein occlusion. In contrast to the full-field ERG the mfERG may be a useful tool to evaluate and monitoring localized cone dysfunction during ischemia-reperfusion.
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