April 2009
Volume 50, Issue 13
ARVO Annual Meeting Abstract  |   April 2009
In-vivo Study of Human Microbial Keratitis With Anterior Segment OCT
Author Affiliations & Notes
  • A. Konstantopoulos
    Ophthalmology, Southampton University Hospitals, Southampton, United Kingdom
  • D. F. Anderson
    Ophthalmology, Southampton University Hospitals, Southampton, United Kingdom
  • P. N. Hossain
    Ophthalmology, Southampton University Hospitals, Southampton, United Kingdom
  • Footnotes
    Commercial Relationships  A. Konstantopoulos, None; D.F. Anderson, None; P.N. Hossain, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 3690. doi:
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      A. Konstantopoulos, D. F. Anderson, P. N. Hossain; In-vivo Study of Human Microbial Keratitis With Anterior Segment OCT. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3690.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Anterior Segment Optical Coherence Tomography (AS-OCT) is a novel imaging modality that provides high-resolution corneal scans by non-contact examination. We investigate microbial keratitis (MK) in a prospective longitudinal study with AS-OCT. We assess the ability of Visante AS-OCT to measure corneal thickness (CT) and infiltrate thickness (IT) in the affected area and investigate the temporal change of these parameters during the course of the disease.

Methods: : Twenty seven patients with suspected microbial keratitis underwent slit-lamp examination and AS-OCT scans on day of presentation and days 3, 7 and 14 post-presentation. The scanning beam passed through the infiltrate centre at a specific meridian for all scans. Measurements of CT and IT were obtained in the centre of the infiltrated area on high-resolution images with calliper tools provided by the Visante OCT software (version 1.1.2). Scans and measurements were carried out by the same investigator.

Results: : Clinical resolution occurred in 26 cases; mean CT decreased from 898m (SD±204) on presentation to 753m (SD±161) [p<0.01], 677m (SD±178) [p<0.001] and 584m (SD±146) [p<0.001] on days 3, 7 and 14 respectively. Mean IT decreased from 394m (SD±182) to 319m (SD±162) [p=0.16], 295m (SD±135) [p=0.06] and 207m (SD±87) [p<0.001] respectively. CT and IT decreased significantly more rapidly (48m/day and 25m/day respectively) in the first 3 days of treatment compared to later time intervals, 3-7 and 7-14 days (p<0.05). In one case with clinical deterioration, CT decreased and IT increased before penetrating keratoplasty was required for perforation.

Conclusions: : This is the first study to measure in-vivo CT and IT during the clinical course of MK. Clinical resolution is associated with a rapid reduction of CT and IT the first 3 days of treatment; CT decreases significantly by day 3, whereas IT only decreases significantly by day 14. AS-OCT provides serial objective assessment of the extent and depth of corneal inflammation.

Keywords: cornea: clinical science • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • inflammation 

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