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Y. Yamauchi, T. Agawa, R. Tsukahara, N. Yamakawa, T. Mizusawa, K. Umazume, H. Goto; Relationship Between Histopathology and High Resolution Optical Coherence Tomography Findings in Rabbits IAA Injected-A Model of Photoreceptor Degeneration. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3797.
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© ARVO (1962-2015); The Authors (2016-present)
Recently, several macular diseases are on discussion of relationship between the IS/OS line evaluated with high resolution optical coherence tomography (OCT) and retinal photoreceptor abnormalities. However, relationship between OCT images and real histopathology of the retina was not well understood. We compared OCT images with histopathology of the retina of photoreceptor degeneration model induced by iodoacetic acid (IAA) in rabbits.
Photoreceptor toxic IAA (20mg/kg) was injected 3 colored rabbits. A total of 6 retinae were submitted for histopathological study following IAA administration, including 2 with 1 day survival and 4 with 4 month survival, as well as 4 healthy control retinae. Before rabbits were euthanized, OCT images were taken with Cirrus HD-OCT TM (Zeiss, Tokyo, Japan).
In controls, IS/OS was demonstrated clear and straight on the OCT imamge. In the rabbit 1day survival after IAA injection, the structure of inner and outer segment of photoreceptor was destroyed histopathologically and IS/OS line was not detected on the OCT image. In the rabbit 4 month survival, retinal pigment epithelium and photoreceptor had disappeared in histopathologically and IS/OS line was not demonstrated on the OCT image. On the other hand, in the rabbit 4 month survival, retinal pigment epithelium and inner segment of photoreceptor was still preserved but the structure of segment was destroyed histopathologically and IS/OS line was detected as a broken and wave line on the OCT image.
IS/OS line of the OCT images reflected histopathology of the inner and outer segment of photoreceptor degeneration rabbit model induced by IAA.
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