April 2009
Volume 50, Issue 13
ARVO Annual Meeting Abstract  |   April 2009
Optical Coherence Tomography in a Murine Retinal Detachment Model
Author Affiliations & Notes
  • C. M. Cebulla
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • M. Ruggeri
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • T. G. Murray
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • E. Hernandez
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • Footnotes
    Commercial Relationships  C.M. Cebulla, None; M. Ruggeri, None; T.G. Murray, None; E. Hernandez, None.
  • Footnotes
    Support  Research to Prevent Blindness
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 3798. doi:
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    • Get Citation

      C. M. Cebulla, M. Ruggeri, T. G. Murray, E. Hernandez; Optical Coherence Tomography in a Murine Retinal Detachment Model. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3798.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Spectral domain optical coherence tomography (SD-OCT) was used to image retinal detachments in vivo, in a murine model of retinal detachment.

Methods: : IACUC approval was obtained. Subretinal injections of 3-4 microliters of healon were delivered to the right eye of six 10-20 week-old mice. Evaluation of the fundus with an operating microscope was performed to document the detachment and fundus photography was performed. In vivo, non-contact, ultra high resolution SD-OCT imaging was performed on day 0, day 2, day 6 and day 14 to evaluate the detachment. Eyes were enucleated for histologic analysis.

Results: : The retinal detachment was confirmed by microscopy in all mice. SD-OCT detected the retinal detachment in 5/6 cases. The retinal detachment was not detected in one eye since it was very peripheral. The retina was partially detached in 3 cases and totally detached in 3 cases. The extent of the retinal detachment was evaluated by measuring the height of the subretinal healon. The retinal layers, including the photoreceptor layer, were evaluated. SD-OCT imaging revealed relative blurring and thickening of the layers of the detached retina on day zero compared to non-detached retina. The maximal elevation of the retinal detachment was difficult to image with SD-OCT on day zero since the height was at the border of the detection limit. Two days post-injection, the retina was mostly re-attached and limited areas of retinal detachment remained. The retinal layers were more clearly defined. SD-OCT imaging was limited on day 6 and 14 by cataract formation. Histologic analysis demonstrated retinal detachments by H & E staining.

Conclusions: : SD-OCT is a promising technology to follow retinal detachment formation and resolution in a murine model.

Keywords: retinal detachment • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) 

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