Purchase this article with an account.
I.-J. Wang, T.-Y. Chen, Y.-F. Shih, L.-K. Lin; The Differential Expression of CYP17 Gene and Sex Steroid Hormone in Myopia. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3829.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
The purpose of our study was to find a single nucleotide polymorphism in the promoter region of CYP17 gene between female and male population and their correlations with serum level of sex steroid hormone and refractive status.
Genomic DNA and serum were collected from 293 subjects (144 females and 149 males) with high myopia (<-6.0 D) and 289 age matched controls (141 females and 148 males) without high myopia. Estradiol, progesterone, testosterone, and free testosterone levels were determined using ELISA kit, which were purchased from Diagnostic Systems Laboratories. And a PCR-RFLP method was used to detect the polymorphism. Associations between the hormone level and various genotypes in case and controls were investigated with Student’s t or chi square test.
Serum estradiol, progesterone and free testosterone level between high myopia cases and normal controls in female subjects were not statistically significant (p=0.1370, p=0.1697 and p=1041), but it has statistical difference in male group (p=0.0001, p=0.0007 and p=3.0683x10-5). Testosterone level between high myopia cases and normal controls show statistical difference in both female and male group (p=3.9121x10-9 and p=0.0002). However, the genotyping success rate was 100%. For CYP17 5'UTR T34C (rs743572), there was no significant difference (p=0.86 and p=0.58) between the high myopia cases and normal controls in female and male groups.
This PDF is available to Subscribers Only