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R. W. Beuerman, A. Barathi, L. Zhou; Quantitative Analysis of the Retina Proteome in a Mouse Model of Myopia. Invest. Ophthalmol. Vis. Sci. 2009;50(13):3830.
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© ARVO (1962-2015); The Authors (2016-present)
Myopia can be induced in the mouse using a spectacle lens procedure. The purpose of this study was to determine if the retina of eyes with experimental myopia show differences in the retina proteome.
Contact lenses were placed over the right eyes of 6 Balb/c mice by attaching a negative (-10D) contact lens over the eye on post-natal day 10. Axial length was measured by AC-Master, OLCI (Carl-Zeiss) and refraction was measured by retinoscopy at 4, 6 and 8 weeks. Mouse retina at 8 weeks was carefully removed from RPE and choroid under a dissecting microscope. Some tissues underwent paraffin embedding to ensure that the retina was removed in± isolation. A quantitative proteomic procedure using iTRAQ (isobaric tags for relative and absolute quantitation) combined with two-dimensional nano-liquid chromatography-nano-electrospray ionization-tandem mass spectrometry to perform a differential analysis of the retina proteome comparing myopic eyes with fellow control eyes. Retina samples used were separately pooled from six eyes with spectacle lens-induced myopia group and the fellow control eyes respectively. ProteinPilot software was used for relative protein quantitation.
The refractions in eyes with lenses were -4.3D ± -1.06D (Mean ±SD) with an axial length of 3.69mm ± 0.07mm while the refraction in fellow eyes was 7.2D ± 1.22D and an axial length of 3.34mm ± 0.05mm. In total, close to 200 proteins were identified in the retina proteome with a 95% confidence, of which 18 proteins were found to be up-regulated (ratio of myopia:contrrol > 1.25) and 10 proteins were down-regulated (ratio of myopia:control < 0.8). Differentially expressed proteins included rhodopsin, delta-aminolevulinic acid dehydratase, acidic leucine-rich nuclear phosphoprotein 32E, crystallin, glyoxalase domain-containing protein 4, retinoschisin, voltage-dependent anion-selective channel protein 3, diazepam binding inhibitor isoform 1, Sod2 superoxide dismutase, creatine kinase M-type, and vimentin. Ontology showed that their biological or molecular functions were associated with phototransduction, cerebellar development and synaptogenesis, retinal development, acyl-CoA binding, response to oxidative stress, energy transduction in tissues, as well as some structural components.
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