April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Expression of S-Opsin-Containing Cells in Degenerative Retina
Author Affiliations & Notes
  • J. Romero del HombreBueno
    Departamento de Biotecnologia, Universidad de Alicante, San Vicente del Raspeig, Spain
  • M. M. Tsai
    Department of Biomedical Engineering, University of Southern California, Los Angeles, California
  • J. De Juan
    Departamento de Biotecnologia, Universidad de Alicante, San Vicente del Raspeig, Spain
  • N. M. Grzywacz
    Department of Biomedical Engineering, University of Southern California, Los Angeles, California
  • E. J. Lee
    Department of Biomedical Engineering, University of Southern California, Los Angeles, California
  • Footnotes
    Commercial Relationships  J. Romero del HombreBueno, None; M.M. Tsai, None; J. De Juan, None; N.M. Grzywacz, None; E.J. Lee, None.
  • Footnotes
    Support  This work was funded by National Eye Institute grants EY11170 and EY016093 to NMG, and by a Rose Hills Foundation Science and Engineering grant, and a James H. Zumberge Research grant to E-JL.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4164. doi:
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      J. Romero del HombreBueno, M. M. Tsai, J. De Juan, N. M. Grzywacz, E. J. Lee; Expression of S-Opsin-Containing Cells in Degenerative Retina. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4164.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : S-opsin is a photo-pigment expressed in short-wavelenght sensitive cones (S-cones) and is essential to support color vision. We investigated the effects of rods death on the distribution of S-opsin-positive cells in retinal-degeneration (RD) rats.

Methods: : For control, Copenhagen rats (Harlan, Indianapolis, IN) of both sexes were used. In turn, we used pigmented S-334ter-line-3 rats, expressing a mutated human-rhodopsin protein, as an RD model. Rats were sacrificed at postnatal (P) days P21, P90, P180, and P600. Control and RD animals were maintained on a daily 12 h light/dark cycle. We studied the morphology, distribution, and identity of S-opsin positive cells in the control and RD rats by immunocytochemistry.

Results: : In control retina, S-opsin immunoreactivity was present in S-cone photoreceptors. However, in RD retinas, from P90 onwards, the somata of S-opsin positive cells were in the outermost part of the inner nuclear layer (INL). Their processes extended toward the outer plexiform layer (OPL). Double-labeling experiments demonstrated that this cell was neither a horizontal cell nor a bipolar cell. Rather, the distal S-opsin cell appears to be a remodeled S-cone photoreceptor, by being arrestin, transducin, and recoverin immunoreactive.

Conclusions: : Our results show that S-cones suffer morphological changes in RD rats. Co-localization of arrestin and transducin in S-opsin positive cells of the RD retina, implies that they may have some phototransduction functionality.

Keywords: retinal degenerations: cell biology • photoreceptors • plasticity 
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