April 2009
Volume 50, Issue 13
ARVO Annual Meeting Abstract  |   April 2009
Holographic Dynamic Control of Neuronal Populations in the Retina
Author Affiliations & Notes
  • N. Farah
    Biomedical Engineering, The Technion - I.I.T., Haifa, Israel
  • I. Reutsky
    Biomedical Engineering, The Technion - I.I.T., Haifa, Israel
  • L. Golan
    Biomedical Engineering, The Technion - I.I.T., Haifa, Israel
  • S. Shoham
    Biomedical Engineering, The Technion - I.I.T., Haifa, Israel
  • Footnotes
    Commercial Relationships  N. Farah, None; I. Reutsky, None; L. Golan, None; S. Shoham, None.
  • Footnotes
    Support  European Research Council Starting Grant #211055
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4225. doi:
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    • Get Citation

      N. Farah, I. Reutsky, L. Golan, S. Shoham; Holographic Dynamic Control of Neuronal Populations in the Retina. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4225.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Development and in-vitro implementation of a novel projection/excitation strategy that can be used to selectively control large retinal neuronal populations, with high temporal precision (msec) and efficient use of light power.

Methods: : Of existing display technologies, digital holographic projection ideally meets these constraints, because the use of phase-modulating spatial light modulators (SLMs) and light diffraction allows an efficient use of input light. Our system directs light from Blue, Green and Red DPSS Lasers onto a Ferroelectric liquid crystal SLM that displays binary holograms. Light patterns were coupled into the camera port of an inverted microscope and projected onto retinas, whose responses were measured using a Multi-Electrode Array (MEA).

Results: : We demonstrate for the first time responses of a population of retinal ganglion cells to patterns of light holographically projected unto wild-type and ChannerhodopsinII transfected retinas. The neurons exhibit spatially-selective responses and have effective receptive fields. We demonstrate sub-millisecond timescale control over the projected light patterns, multi-wavelength excitation, and computational strategies that eliminate the effect of speckle.

Conclusions: : High-rate holographic projection was demonstrated as an enabling photo-stimulation modality towards the development of a retina neuro-prosthetic, theoretically capable of eliciting over 1 million spikes per second, with millisecond timing precision. Our system can also be applied in experimental studies of the visual system requiring ultra-high-rate stimulus control.

Keywords: retina • ganglion cells 

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