April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Presence of SLURP-1 and Nicotinic Acetylcholine Receptor in the Rabbit Lacrimal Gland
Author Affiliations & Notes
  • S. K. Carlsson
    Dept of Pure and Applied Natural Sciences, University of Kalmar, Kalmar, Sweden
  • A. Pettersson
    Dept of Pure and Applied Natural Sciences, University of Kalmar, Kalmar, Sweden
  • J. P. Gierow
    Dept of Pure and Applied Natural Sciences, University of Kalmar, Kalmar, Sweden
  • D. Delbro
    Dept of Chemistry and Biomedical Sciences, University of Karlstad, Karlstad, Sweden
  • Footnotes
    Commercial Relationships  S.K. Carlsson, None; A. Pettersson, None; J.P. Gierow, None; D. Delbro, None.
  • Footnotes
    Support  Crownprincess Margareta's Eye Research Foundation (JPG) and University of Kalmar Faculty Research Grant (DD & JPG)
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4242. doi:
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      S. K. Carlsson, A. Pettersson, J. P. Gierow, D. Delbro; Presence of SLURP-1 and Nicotinic Acetylcholine Receptor in the Rabbit Lacrimal Gland. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4242.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : It has recently been shown that nicotinic acetylcholine receptors are present also in non-neuronal tissue and that they together with SLURP (secreted mammalian Ly-6/urokinase plasminogen acivator receptor-related protein) might be involved in immuno-modulatory/apoptotic events. The aim of the present study is therefore to investigate the presence of the alpha-7 subunit of the nicotinic acetylcholine receptor and SLURP in the rabbit lacrimal gland.

Methods: : Rabbit lacrimal gland acinar cells were prepared according to our standard procedure (Gierow et al., Am. J. Physiol. (Cell Physiology) 271: C1685, 1996; Andersson et al, Glycobiol. 15: 211, 2005) yielding single cells that were placed in a serum-free culture medium on standard culture plates coated with Matrigel for two days. The cultured cells were rinsed briefly and secretion was measured after a pre-incubation for 30 min at 37 C, with buffer alone, followed by incubation for an additional 30 min w./w.o. secretagogues as indicated. Basal secretion and regulated secretion was determined enzymatically as secreted beta-hexosaminidase activity. The lacrimal gland was also subjected to immunohistochemistry to confirm the presence the alpha-7 subunit, SLURP-1 and -2.

Results: : Secretion of beta-hexosaminidase from cultured acinar cells was significantly increased by approximately 30 %, at 1 µM nicotine. Immunohistochemistry indicated that both the alpha-7 subunit of the nicotinic acetylcholine receptor and its ligand SLURP-1 are present in lacrimal tissue, whereas SLURP-2 could not be detected.

Conclusions: : Our results indicate that both the alpha-7 subunit of the nicotinic acetylcholine receptor and its ligand SLURP-1 are present in the rabbit lacrimal gland and that nicotine tartrate has stimulatory effects on protein secretion in cultured cells.

Keywords: lacrimal gland • neurotransmitters/neurotransmitter systems • receptors 
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