April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Abnormal Epithelial Differentiation and Tear Film Alteration in Pinguecula
N. Dong; W. Li; W. Qin; H. Lin; C. Li; W. Chen; Z. Liu
Author Affiliations & Notes
  • N. Dong
    affiliated Xiamen Eye Center, Eye Institute of Xiamen University, Xiamen, China
  • W. Li
    affiliated Xiamen Eye Center, Eye Institute of Xiamen University, Xiamen, China
  • W. Qin
    Radiotherapy department, Zhongshan hospital of Xiamen University, Xiamen, China
  • H. Lin
    affiliated Xiamen Eye Center, Eye Institute of Xiamen University, Xiamen, China
  • C. Li
    affiliated Xiamen Eye Center, Eye Institute of Xiamen University, Xiamen, China
  • W. Chen
    affiliated Xiamen Eye Center, Eye Institute of Xiamen University, Xiamen, China
  • Z. Liu
    affiliated Xiamen Eye Center, Eye Institute of Xiamen University, Xiamen, China
  • Footnotes
    Commercial Relationships  N. Dong, None; W. Li, None; W. Qin, None; H. Lin, None; C. Li, None; W. Chen, None; Z. Liu, None.
  • Footnotes
    Support  National Natural Science Foundation of China (No. 30872809); the National High-tech Research and Development Program (863 Program) of China (No. 2006AA02A131)
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4275. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Abnormal Epithelial Differentiation and Tear Film Alteration in Pinguecula
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      N. Dong, W. Li, W. Qin, H. Lin, C. Li, W. Chen, Z. Liu; Abnormal Epithelial Differentiation and Tear Film Alteration in Pinguecula. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4275.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Purpose: : To investigate differentiation of conjunctival epithelium and tear film function in pingueculae.

Methods: : Twelve patients (12 eyes), who underwent simple excision for pingueculae, were enrolled in this study. Immunostaining_K14, K19, K10, MUC5AC, Pax6, P63, Ki67, and K16_was performed on pinguecula epithelium and normal conjunctival epithelium. The Schirmer I test as well as the tear film break-up time (TFBUT) were evaluated just prior to and 1 month after surgery.

Results: : Abnormal epidermal differentiation of pinguecula tissue, as evidenced by a decline in or absence of Pax6 expression, was accompanied by a decline in or absence of K19 keratin and MUC5AC, and an increase of in K10 and K14 keratin. Furthermore, pinguecula epithelium was actively proliferating, as evidenced by positive expression of Ki67, P63, and K16 keratin. The Schirmer I test did not indicate any significant differences pre- and post-operatively. However, the TFBUT was significantly prolonged 1 month post surgery compared to pre-surgery.

Conclusions: : Pinguecula is a condition of abnormal epithelial differentiation. It is characterized by squamous proliferation and metaplasia, resulting in instability of tear film with normal basic tear secretion.

Keywords: conjunctiva • differentiation • immunohistochemistry 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept