April 2009
Volume 50, Issue 13
ARVO Annual Meeting Abstract  |   April 2009
Immunohistochemical Differentiation of Melanocytic Conjunctival Tumors
Author Affiliations & Notes
  • E. M. Messmer
    Dept of Ophthalmology, Ludwig Maximilians University, Munich, Germany
  • U. B. Grenot
    Dept of Ophthalmology, Ludwig Maximilians University, Munich, Germany
  • A. Kampik
    Dept of Ophthalmology, Ludwig Maximilians University, Munich, Germany
  • Footnotes
    Commercial Relationships  E.M. Messmer, None; U.B. Grenot, None; A. Kampik, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4281. doi:
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    • Get Citation

      E. M. Messmer, U. B. Grenot, A. Kampik; Immunohistochemical Differentiation of Melanocytic Conjunctival Tumors. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4281.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To differentiate pigmented lesions of the conjunctiva using immunohistochemical markers.

Methods: : Fifty-three conjunctival nevi, 10 primary acquired melanoses (PAM) without atypia, 25 PAM with atypia, and 24 conjunctival melanomas were analyzed by immunohistochemistry using the alkaline-phosphatase anti-alkaline phosphatase method. Antibodies against melanocytic cells (S-100, HMB-45, Melan-A), t-lymphocytes (anti-CD3), macrophages (anti-CD68), and the proliferation marker Ki-67 were applied. Immunohistochemical staining was assessed semi-quantitatively according to intensity and number of positively staining cells using a grading scale from 0 to 3. Fisher’s exact tests and Mann Whitney tests were employed for statistical analysis.

Results: : PAM with and without atypia showed significantly different staining for S-100 (p=0.027), HMB-45 (p=0.004), Melan-A (p=0.007), CD3 (p=0.019) and CD68 (p=0.015). Ki-67 expression (p=0.015) and the amount of t-lymphocytes (p=0.018) were significantly higher in conjunctival melanomas compared to PAM with atypia.

Conclusions: : In addition to cytological characteristics, benign, potentially malignant, and malignant pigmented conjunctival tumors can be differentiated according to their immunohistochemical staining pattern for melanocytic and inflammatory markers. Therefore, immunohistochemistry is a valuable tool in the differentiation of histologically questionable lesions.

Keywords: conjunctiva • tumors • immunohistochemistry 

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