Purchase this article with an account.
E. M. Messmer, U. B. Grenot, A. Kampik; Immunohistochemical Differentiation of Melanocytic Conjunctival Tumors. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4281.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To differentiate pigmented lesions of the conjunctiva using immunohistochemical markers.
Fifty-three conjunctival nevi, 10 primary acquired melanoses (PAM) without atypia, 25 PAM with atypia, and 24 conjunctival melanomas were analyzed by immunohistochemistry using the alkaline-phosphatase anti-alkaline phosphatase method. Antibodies against melanocytic cells (S-100, HMB-45, Melan-A), t-lymphocytes (anti-CD3), macrophages (anti-CD68), and the proliferation marker Ki-67 were applied. Immunohistochemical staining was assessed semi-quantitatively according to intensity and number of positively staining cells using a grading scale from 0 to 3. Fisher’s exact tests and Mann Whitney tests were employed for statistical analysis.
PAM with and without atypia showed significantly different staining for S-100 (p=0.027), HMB-45 (p=0.004), Melan-A (p=0.007), CD3 (p=0.019) and CD68 (p=0.015). Ki-67 expression (p=0.015) and the amount of t-lymphocytes (p=0.018) were significantly higher in conjunctival melanomas compared to PAM with atypia.
In addition to cytological characteristics, benign, potentially malignant, and malignant pigmented conjunctival tumors can be differentiated according to their immunohistochemical staining pattern for melanocytic and inflammatory markers. Therefore, immunohistochemistry is a valuable tool in the differentiation of histologically questionable lesions.
This PDF is available to Subscribers Only