Purchase this article with an account.
H. Keino, Y. Sato, K. Umezawa, A. A. Okada; Dehydroxymethylepoxyquinomicin (DHMEQ) Inhibits TNF-Alpha Induced Expression of ICAM-1, IL-8 and MCP-1 in Human Retinal Pigment Epithelial Cells in vitro. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4284.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Dehydroxymethylepoxyquinomicin (DHMEQ) derived from the structure of an antibiotic epoxyquinomicin C is a novel nuclear factor (NF) kappa B inhibitor. We investigated whether DHMEQ can modulate the proinflammatory cytokine induced expression of intracellular adhesion molecule (ICAM)-1 and various chemokines in human retinal pigment epithelial (RPE) cells.
Cultures of ARPE-19 cells were exposed to tumor necrosis factor (TNF)-alpha, and ICAM-1 expression was measured by flow cytometry. Production of interleukin (IL)-8 and monocyte chemoattractant protein (MCP)-1 were assessed by enzyme linked immunosorbent assay (ELISA).
TNF-alpha increased the expression of ICAM-1 at the protein level in ARPE-19 cells, and DHMEQ effectively downregulated this expression. Furthermore, DHMEQ suppressed the production of both IL-8 and MCP-1 in TNF-alpha stimulated ARPE-19 cells.
DHMEQ inhibited the expression of ICAM-1 and the inflammatory chemokines IL-8 and MCP-1 in ARPE-19 cells stimulated with TNF-alpha. This suggests that DHMEQ may play a role in reducing ICAM-1 and chemokine mediated inflammatory disorders in the eye.
This PDF is available to Subscribers Only