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R. Jun, K.-E. Han; In vivo Effects of Fourth-Generation Fluoroquinolones on Rabbit Corneal Keratocytes. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4552.
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to investigate the in vivo effect of fourth-generation fluoroquinolones(moxifloxacin and gatifloxacin) on rabbit corneal keratocyte
Fifty six New Zealand white rabbits were randomly divided into 4 groups of 14 rabbits per group: gatifloxacin 0.3%, moxifloxacin 0.5%, levofloxacin 0.5% or preservative-free artificial tear. In all rabbits a 12-mm-diameter epithelial wound was mechanically made to the center of the right cornea. The left eye of each rabbit served as an intact control. All rabbits received one drop of the test eye drops every hour for 48 hours followed by every 2 hours for an additional 48 hours. The rabbits were sacrificed at 96 hours. Transforming growth factor(TGF)-β1 and interleukin(IL)-1 expression were examined using real-time polymerase chain reaction. The expression of matrix metalloproteinase(MMP)-1, 2, 8 and 9 was evaluated by western blot assay and zymography. The changes of structure of the cells were evaluated with transmission electron microscopy.
The expression of TGF- β1 was similar in both debrided and intact cornea in all test groups. IL-1 was highly detected in debrided cornea than intact cornea, especially in the gatifloxacin, moxifloxacin and artificial tear groups. MMP-1, 2, 8 and MMP-9 were detected in all intact and debrided corneas. MMP-1 and 2 showed significantly increased expressions in the gatifloxacin group than the moxifloxacin group. All the tested MMPs were expressed greater in the gatifloxacin group than the levofloxacin group. In the levofloxacin group, expressions of the all MMPs were lowest in the test groups. Cytotoxic changes of keratocytes were detected more in the moxifloxacin group than in any other test groups.
The fourth generation fluoroquinolones induce more expressions of IL-1 and MMPs and structural changes than third generation fluoroquinolone or artificial tear in the rabbit cornea.
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