April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
In-vitro and in-vivo Evaluation of Penetrating Microelectrode Array for Optic-Nerve Visual Prosthesis
X. Sui; Y. Shao; T. Liang; F. Tan; Z. Han; X. Chai; Q. Ren
Author Affiliations & Notes
  • X. Sui
    Institute for Laser Medicine and Biophotonics, Department of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
  • Y. Shao
    Institute for Laser Medicine and Biophotonics, Department of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
  • T. Liang
    Institute for Laser Medicine and Biophotonics, Department of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
  • F. Tan
    Institute for Laser Medicine and Biophotonics, Department of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
  • Z. Han
    Institute for Laser Medicine and Biophotonics, Department of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
  • X. Chai
    Institute for Laser Medicine and Biophotonics, Department of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
  • Q. Ren
    Institute for Laser Medicine and Biophotonics, Department of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
  • Footnotes
    Commercial Relationships  X. Sui, None; Y. Shao, None; T. Liang, None; F. Tan, None; Z. Han, None; X. Chai, None; Q. Ren, None.
  • Footnotes
    Support  the National Basic Research Program of China (973 Program, 2005CB724302), and the National Natural Science Foundation of China for the Youth (30700217)
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4586. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      In-vitro and in-vivo Evaluation of Penetrating Microelectrode Array for Optic-Nerve Visual Prosthesis
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      X. Sui, Y. Shao, T. Liang, F. Tan, Z. Han, X. Chai, Q. Ren; In-vitro and in-vivo Evaluation of Penetrating Microelectrode Array for Optic-Nerve Visual Prosthesis. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4586.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Purpose: : To evaluate the in-vitro electrochemical characteristics and in-vivo experiment of multi-channel Pt/Ir microelectrode array for optic nerve visual prosthesis.

Methods: : A five-channel Platinum/Iridium (Pt/Ir: 70%/30%) microelectrode array was custom-designed (Micro Probe Inc) for intraorbital optic nerve stimulation. The metal shafts were 125µm in diameter, spaced 400µm apart from the center part and mounted step-wisely on a base plate with 1.75mm in length and 0.5mm in width. Height of five different shafts ranged from 0.9mm to 1.5mm with 0.15mm increased step. Each shaft was insulated by Parylene-C with penetrating tip exposed. The impedance of the microelectrode array was measured by the Agilent E4980A Precision LCR Meter in physiological saline solution at room temperature with three electrodes: the working electrode, a flat platinum counter electrode, and an Ag/AgCl reference electrode with the amplitude of 50 mVpp. Charge injection capacity of the microelectrode array was measured by current pulse stimulation with the three-electrode system. Symmetric cathodal-first, charge- balanced biphasic pulses of different amplitudes with time duration from 72 µs to 504 µs were applied without bias potential applied. Besides, the proper implantation surgery was applied for in-vivo experiment with stimuli of different parameters.

Results: : It was shown that five shafts of the microelectrode had uniform impedance-frequency and phase-frequency characteristics and the typical impedance value was about 10±0.5kΩ at 1 kHz. The voltage response of current pulse stimulation of the array indicated the charge-injection capacity was 200±20µC/cm2. The in-vivo acute animal experiment showed that the electrical evoked potentials (EEPs) can be recorded from primary visual cortex.

Conclusions: : The in-vitro electrochemical measurement indicated the uniform impedance and desirable charge injection capacity of the microelectrode array. The in-vivo animal experiment was applied and relevant EEPs were recorded. Study results suggested that the microelectrode array satisfied the experiment requirements.

Keywords: electrophysiology: non-clinical • neuro-ophthalmology: optic nerve • optic nerve 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept