April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Influence of Oral Supplementation of Omega-3 and Omega-6 Fatty Acids on Conjunctival Inflammatory Markers in Dry Eye Patients
Author Affiliations & Notes
  • C. P. Garcher
    Ophthalmology, University Hospital, Dijon, France
  • F. Brignole-Baudouin
    Toxicology, Paris Descartes University, Paris, France
  • C. Baudouin
    Ophthalmology, CHNO XV-XX, Paris, France
  • P. Aragona
    Ophthalmology, University Hospital, Messina, Italy
  • M. Rolando
    Ophthalmology, University Hospital, Genoa, Italy
  • M. Labetoulle
    Ophthalmology, University Hospital, Kremlin Bicêtre Paris, France
  • P.-J. Pisella
    Ophthalmology, University Hospital, Tours, France
  • R. Siou-Mermet
    Bausch & Lomb, Montpellier, France
  • Footnotes
    Commercial Relationships  C.P. Garcher, None; F. Brignole-Baudouin, None; C. Baudouin, None; P. Aragona, None; M. Rolando, None; M. Labetoulle, None; P.-J. Pisella, None; R. Siou-Mermet, Bausch & Lomb, E.
  • Footnotes
    Support  Bausch & Lomb
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4638. doi:
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      C. P. Garcher, F. Brignole-Baudouin, C. Baudouin, P. Aragona, M. Rolando, M. Labetoulle, P.-J. Pisella, R. Siou-Mermet; Influence of Oral Supplementation of Omega-3 and Omega-6 Fatty Acids on Conjunctival Inflammatory Markers in Dry Eye Patients. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4638.

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      © ARVO (1962-2015); The Authors (2016-present)

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  • Supplements
Abstract

Purpose: : Some studies have suggested that the intake of omega-3 or omega-6 fatty acids may improve dry eye syndrome. This study was designed to assess whether the oral supplementation of omega-3 and omega-6 fatty acids can reduce inflammatory markers in the conjunctiva of dry eye patients.

Methods: : 138 patients presenting mild to moderate dry eye were included in a 3-month, multicenter, double-masked, randomized, controlled clinical study. They were assigned to either placebo capsules (medium chain triglycerides) or capsules containing omega-3 fatty acids (855mg), omega-6 fatty acids (15mg), vitamins (C, E, B6, B12) and zinc. Impression cytology was performed on the worst eye at inclusion and at Month 3 to assess the rate of positive cells expressing HLA-DR inflammatory marker and their fluorescence intensity. Other assessments included a composite score for ocular dry eye symptoms and objective signs.

Results: : 121 subjects had available impression cytology and 106 were compliant with the protocol. Both populations were analyzed. The reduction of the percentage of HLA-DR positive cells was more important in the supplemented group of the 106 subjects (p=0.021). Furthermore, the reduction of the fluorescence intensity was also greater in the supplemented group of the 106 subjects (p=0.017) and of the 121 subjects (p=0.041). For symptoms and signs, there was a better but not significant improvement of burning, dryness and stinging and of the lissamine and corneal fluorescein stainings in the supplemented group.

Conclusions: : This study suggests that a supplementation of omega-3 and omega-6 fatty acids in dry eye patients reduces the HLA-DR conjunctival inflammatory markers. No significant difference was found for the signs and symptoms but there was a consistent trend for a better improvement in the supplemented group.

Clinical Trial: : www.ISRCTN.org 17233445

Keywords: conjunctiva • inflammation • clinical (human) or epidemiologic studies: treatment/prevention assessment/controlled clinical trials 
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