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J.-M. Seo, H. Shin, E. Kim, K. Koo, E. Shin, G. J. Suaning, N. H. Lovell, D.-I. Cho, S. Kim, H. Chung; Interpretation of Optical Coherent Tomographic Findings in the Rabbit Eye with Implanted Retinal Prosthesis Electrode Arrays. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4745.
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© ARVO (1962-2015); The Authors (2016-present)
Optical coherent tomography (OCT) is a useful method to evaluate the cross-sectional morphology of the retina in vivo. However, the image processing algorithms used in OCT may generate incorrect images from interferometric data. Implanted microelectrode arrays (MEAs) for retinal stimulation were evaluated by OCT and the acquired images were compared with the real dimensional configuration of the MEAs in vivo.
New Zealand white rabbits (N=10) weighing 3.0 kg were used for in vivo biocompatibility evaluation of the MEA. Three types of planar MEA (polyimide-based gold electrode, polyimide/silicone-based gold electrode, silicone-based platinum electrode) and two types of polyimide-based 3D MEA (mushroom-shaped gold electrode, arrowhead-shaped gold electrode) were implanted into the subretinal or suprachoroidal space of the rabbit eyes. Spectral domain OCT (CirrusTM) was taken serially for one month and the results were compared with data from cross-sectional microscopic morphology and histology.
All the electrodes were implanted safely in rabbit eyes. Silicone showed lower reflectance than polyimide, and the polyimide gave lower reflectance than the retinal pigment epithelium but higher reflectance than the inner nuclear layer. Metallic electrodes generated various reflectances with posterior shadow. Instead of revealing indentation or elevation of the retina over the electrode, OCT showed a straight internal limiting membrane line, and paradoxical posterior indentation or deflection of the sclera. Cross-sectional microscopic morphology and the histology confirmed the indentation or elevation of the retina over the electrode.
Although OCT was a good method to show the relationship between the retina and the implanted MEA, careful interpretation should be made in evaluating in vivo status of MEA in the subretinal or suprachoroidal space of the eye.
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