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M. Tauchi, T. A. Fuchs, D. F. Woodward, E. Lütjen-Drecoll; Bimatoprost-Induced Hypertrichosis of Eyelashes: Murine Model. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4820.
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To establish a mouse model and investigate the mechanism of bimatoprost-induced eyelash hypertrichosis.
We developed a murine model using upper eyelids of C57/BL6J female mice. Serial, 1µm, semi-thin sections were prepared from Ito-fixed, epon embedded eyelids, and morphology of eyelid and eyelash was observed. Bimatoprost (3µl; LumiganTM ) and its vehicle were topically applied to the eyes of 4-week old mice, once daily for 14 days (n=10 for each). After treatment, total eyelashes were counted under a stereomicroscope. In each eyelid, thickness of eyelashes from 3 different length groups was estimated using image processing software. Furthermore, the eyelids were processed as described above, and morphological changes were investigated in serial semi-thin sections (160 sections/ eyelid), the growth cycle phase was analysed, and the number of follicles was quantified.
The overall morphology of mouse eyelid was comparable to that of humans. Eyelash number was 0 (newborn) and increased to ~140 in the 3rd week, and stayed the same until the 6th week. Eyelash morphogenesis progressed faster than skin hairs, followed by the growth cycle in a synchronized manner at the beginning. After 2 weeks of treatment, bimatoprost-treated eyelids appeared to have significantly more eyelashes compared to contralateral or vehicle-treated eyelids. This was not accompanied by increased eyelash follicles when examined in histological sections. Medium and short eyelashes, but not long eyelashes, were thicker in the bimatoprost-treated group compared to contralateral eyes or the vehicle-treated group. Bimatoprost did not cause morphological abnormalities in the eyelash follicles but the bulb diameter became greater. In addition, bimatoprost increased the duration of anagen, a growing phase, compared to vehicle.
Bimatoprost increased the number, thickness, and length of eyelashes in mice similar to the side effects seen in human glaucoma patients on bimatoprost therapy. Our results suggest that bimatoprost affects eyelash growth cycle regulation, especially the anagen phase.
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