April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Gene Therapy for Corneal Neovasucalzation Using Polyplex Micelle
T. Usui; A. Iriyama; Y. Yanagi; Y. Tamaki; S. Amano; N. Nishiyama; K. Kataoka
Author Affiliations & Notes
  • T. Usui
    Ophthalmology, Univ of Tokyo Sch of Med, Tokyo, Japan
  • A. Iriyama
    Ophthalmology, Univ of Tokyo Sch of Med, Tokyo, Japan
  • Y. Yanagi
    Ophthalmology, Univ of Tokyo Sch of Med, Tokyo, Japan
  • Y. Tamaki
    Ophthalmology, Univ of Tokyo Sch of Med, Tokyo, Japan
  • S. Amano
    Ophthalmology, Univ of Tokyo Sch of Med, Tokyo, Japan
  • N. Nishiyama
    Materials Engineering, Univ of Tokyo, Tokyo, Japan
  • K. Kataoka
    Materials Engineering, Univ of Tokyo, Tokyo, Japan
  • Footnotes
    Commercial Relationships  T. Usui, None; A. Iriyama, None; Y. Yanagi, None; Y. Tamaki, None; S. Amano, None; N. Nishiyama, None; K. Kataoka, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4964. doi:
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      T. Usui, A. Iriyama, Y. Yanagi, Y. Tamaki, S. Amano, N. Nishiyama, K. Kataoka; Gene Therapy for Corneal Neovasucalzation Using Polyplex Micelle. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4964.

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Abstract

Purpose: : To investigate the gene delivery of nonviral vector encapsulating polyplex micelle and inhibitory effects of angiogenesis using soluble flt-1 plasmid encapsulating polyplex micelle in mice corneal neovascularization models.

Methods: : A block copolymer, poly(ethylene glycol) (PEG)-block-polycation, carrying ethylenediamine units in the side chain (PEG-b-P[Asp(DET)]) was prepared. PEG-b-P[Asp(DET)] formed a polyplex micelle through polyion complex formation with plasmid DNA. To evaluate in vivo gene transfer efficiency, PEG-b-P[Asp(DET)] micelle was injected into mice subconjunctiva and expression of the reporter gene was assessed. Polyplexes from homopolymer P[Asp(DET)] was used for control. Further, the PEG-b-P[Asp(DET)] polypolex micelle with expression plasmid vector of soluble flt-1 was injected in mice corneal neovascularization models.

Results: : Subconjunctival injection of the PEG-b-P[Asp(DET)] polypolex micelle with reporter gene showed prolonged gene expression with low cytotoxicity. The polypolex micelle with soluble flt-1plasmid gene transfer into subconjunctival space showed significant inhibition of corneal neovascularization.

Keywords: cornea: basic science • neovascularization • gene transfer/gene therapy 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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