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T. Usui, A. Iriyama, Y. Yanagi, Y. Tamaki, S. Amano, N. Nishiyama, K. Kataoka; Gene Therapy for Corneal Neovasucalzation Using Polyplex Micelle. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4964.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the gene delivery of nonviral vector encapsulating polyplex micelle and inhibitory effects of angiogenesis using soluble flt-1 plasmid encapsulating polyplex micelle in mice corneal neovascularization models.
A block copolymer, poly(ethylene glycol) (PEG)-block-polycation, carrying ethylenediamine units in the side chain (PEG-b-P[Asp(DET)]) was prepared. PEG-b-P[Asp(DET)] formed a polyplex micelle through polyion complex formation with plasmid DNA. To evaluate in vivo gene transfer efficiency, PEG-b-P[Asp(DET)] micelle was injected into mice subconjunctiva and expression of the reporter gene was assessed. Polyplexes from homopolymer P[Asp(DET)] was used for control. Further, the PEG-b-P[Asp(DET)] polypolex micelle with expression plasmid vector of soluble flt-1 was injected in mice corneal neovascularization models.
Subconjunctival injection of the PEG-b-P[Asp(DET)] polypolex micelle with reporter gene showed prolonged gene expression with low cytotoxicity. The polypolex micelle with soluble flt-1plasmid gene transfer into subconjunctival space showed significant inhibition of corneal neovascularization.
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