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J. G. Conrath, F. Matonti, B. Ridings, F. Chavane, G. S. Masson, L. Hoffart; Inhibition of Corneal Neovascularization: Comparison of Different Doses of Bevacizumab Versus Dexamethasone in an Experimental Rat Model. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4969.
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To compare the inhibitory effects of different doses of bevacizumab with both saline and dexamethasone on the inflammatory angiogenesis in the rat cornea induced by small chemical lesions.
Chemical cauterization of cornea was performed on both eyes in 20 rats. Animals were then divided randomly in 5 groups and received, on one eye, a daily subconjunctival injection during 7 consecutive days of one of these 5 treatments: balanced salt solution (BSS); dexamethasone phosphate 0.4mg/day; bevacizumab 2.5mg/day, 3.75mg/day or 5.0mg/day. In all animals, the fellow eye received a subconjunctival injection of BSS. Clinical examination under slit lamp was performed daily for 7 days to evaluate corneal opacity and vessel size evolution. Computer-assisted quantitative image analysis was used to measure the total corneal area covered by neovascularization.
At final examination, the dexamethasone group showed a significant lowering in corneal opacity score as compared to both control and bevacizumab 2.5mg/day groups (p=0.025; p=0.007). On the new vessels size score, dexamethasone group showed a significant reduction as compared to all other groups (p=0.013). Surface of corneal neovascularization was significantly smaller in dexamethasone and bevacizumab 5.0mg/day groups compared to control (p=0.043 and p=0.049 respectively) and bevacizumab 2.5mg/day groups (p=0 .016 and, p=0 .014 respectively).
Our study demonstrates the ability of a 5.0mg/day bevacizumab subconjunctival injection to cause a short-term involution of corneal neovascularization after corneal alkali burn but with limited effects compared to steroids. Further studies using combinations of these treatments are needed.
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