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J. E. Gallo, J. E. Mancini, J. O. Croxatto; Purinergic Receptors Are Up-regulated in the Retina of a Rat Model of Type 1 Diabetes. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4994.
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To compare the expression of purinergic P2Y2 and P2X2 receptors in the retina of a type 1 diabetes model.
Twenty male Wistar rats of 250gr were treated with an intraperitoneal injection (IP) of 45mg/kg of streptozotocin (STZ). Animals with glycemia levels above 200mg/dl were included in the study. Ten out of 20 diabetic rats received an IP injection of suramine. A control group was constituted by 10 rats without diabetes. Animals were killed after 37 weeks of diabetes. Cross sections of retinas were analyzed by immunofluorescence using glial fibrillary acidic protein (GFAP), P2Y2 and P2X2 receptors antibodies.
Immunostaining of P2Y2, P2X2 and GFAP was found higher in diabetics than in controls. Pattern igual en diabet and controls, but An increase of the expression of P2Y2 in the diabetic animals in ganglion cells layer and in internal nuclear layer was the same with the 2 methods and confirmed with western blot . P2X2 immuno expression was higher in DBT animals in the fibrilar layer. Diabetic animals treated with suramine showed less expression of P2Y2, P2X2 and GFAP immune staining.
Results may contribute to new insights in the development of retinopathy in experimental diabetes. The purinergic pathway should be further analysed.
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