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L. Zhang, L. Xiao, Y. Li, J. Liu, S. Sun, L. Chen, W.-B. Liu, Y.-M. Xiao, Y. Li, D. W. Li; AKT/PKB Pathway Components in the Mouse Eye. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4365.
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The AKT/PKB signal transduction pathway regulates many biological processes including transcription, cell cycle progression, metabolism, and apoptosis. Increasing studies implicate that AKT signal transduction pathway plays a pivotal role in the development of the ocular eye tissues. In present study, we have examined the expression patterns of the three members of the Akt family and their related signaling components including Raf, PTEN, PDK1 and Gsk3beta1 in the ocular tissues of the adult mouse eye.
RT-PCR was used for exploration of the mRNA levels. Western blot analysis was used for detecting the protein levels and immunocytochemistry was used to localize the protein expression.
The proteins for AKT1 or AKT2 are more abundant than that for AKT3. AKT1 and AKT2 display similar expression patterns in the retina, lens epithelium and cornea but show much reduced levels in the lens fiber. In contrast, AKT3 expression is only confined to retina. The mRNAs for three AKT isoforms are highly expressed in the retina and lens fiber but display a marked decrease in lens epithelium. Active AKT (phosphorylated at T-450, S-473 and T-308) is mainly located in the retina, lens epithelium and cornea. The active (phosphorylated) forms of Raf9(Ser259), PTEN(Ser380), PDK1(Ser241) and Gsk3beta(Ser9) display similar patterns in these tissues but are much reduced in the lens fiber cells.
Out results suggest that AKT/PKB family members and their related components are differentially expressed in the ocular tissues of mouse eye. The AKT-PKB pathway plays an important role in mouse eye.
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