April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
A 2nd Generation Perfluorocarbon Liquids: In vivo Biocompatibility Study
M. C. Aguilar; E. Arrieta; C. Medina; E. Hernandez; S. Dubovy; J.-M. Parel
Author Affiliations & Notes
  • M. C. Aguilar
    Dept. of Ophthalmology, Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine, Miami, Florida
  • E. Arrieta
    Dept. of Ophthalmology, Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine, Miami, Florida
  • C. Medina
    Dept. of Ophthalmology, Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine, Miami, Florida
  • E. Hernandez
    Dept. of Ophthalmology, Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine, Miami, Florida
  • S. Dubovy
    Dept. of Ophthalmology, Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine, Miami, Florida
  • J.-M. Parel
    Dept. of Ophthalmology, Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine, Miami, Florida
    Dept. of Biomedical Engineering, College of Engineering, Univ. of Miami, Coral Gables, Florida
  • Footnotes
    Commercial Relationships  M.C. Aguilar, None; E. Arrieta, None; C. Medina, None; E. Hernandez, None; S. Dubovy, None; J.-M. Parel, None.
  • Footnotes
    Support  Florida Lions Eye Bank; Abeamed Inc; NIH Center Grant P30EY14801; an unrestricted grant from Research to Prevent Blindness; Henri and Flore Lesieur Foundation (JMP)
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4447. doi:
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      M. C. Aguilar, E. Arrieta, C. Medina, E. Hernandez, S. Dubovy, J.-M. Parel; A 2nd Generation Perfluorocarbon Liquids: In vivo Biocompatibility Study. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4447.

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Abstract

Purpose: : To assess biocompatibility and stability of a 2nd generation novel vitreous substitutes.

Methods: : 5 adult rabbits (4 NZW and 1 Pigmented Dutch Belted) were used for this blind study (the authors were not aware of the polymer composition until the end of the study). 3 novel PFCL polymers (Al.Chi.Mi.A, SLR, Italy) labeled AL18, GB20 and PC12 and a FDA approved control polymer (Okta-line (Bausch & Lomb, Rochester, NY) were tested. Partial vitreous aspiration/substitution was performed under deep IM anesthesia: a 180o degree peritomy was made, a SuperQuad fundus contact lens was placed against the cornea, a 23ga needle was used to manually aspirate ~0.5cc of vitreous, the track was closed with a 8-0 Vicryl mattress. ~0.5cc of polymer was injected via 27ga needle held above the retina. The conjunctiva was closed with 10-0 nylon. The rabbits were examined at POD 1 and 3 by photo slit-lamp and at POD 7, 14, 21, 28, 35, 42, 60, 90, and 120 under the operation microscope and by RetCAM imaging. Histological analysis was performed immediately after euthanasia on all operated and control eyes. Then the composition of the polymers was revealed to the authors.

Results: : There were no clinical signs of infection; pachymetry and intraocular pressure remained normal in all rabbits throughout the study. Focal lens opacities slowly developed with time in all animals except for the control polymer which was totally emulsified by POD 5. Histology analyses revealed that test polymers PC12 (C9H11F9O, d=1.1562g/ml, n=1.3255) and GB20 (C11H15F9O, d=1.2337g/ml, n=1.3435), caused a mild retinal inflammation; they also slowly emulsified during the course the study. AL18 polymer (C11H11F13O, d=1.3498g/ml, n=1.3245) remained as a single large bubble on the retina throughout the study and histology showed the retinal morphology to be normal when compared to the control eye. No cavities were found in any of the ocular tissues.

Keywords: vitreous substitutes • vitreoretinal surgery • vitreous 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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