April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Immunohistochemical Study of Diamond-Dusted Tano Scratchers After Macular Surgery
R. Somani; S. R. Russell; J. C. Folk; H. C. Boldt; K. M. Gehrs; R. F. Mullins; V. B. Mahajan
Author Affiliations & Notes
  • R. Somani
    Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa
  • S. R. Russell
    Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa
  • J. C. Folk
    Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa
  • H. C. Boldt
    Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa
  • K. M. Gehrs
    Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa
  • R. F. Mullins
    Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa
  • V. B. Mahajan
    Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa
    Omics Laboratory, Iowa City, Iowa
  • Footnotes
    Commercial Relationships  R. Somani, None; S.R. Russell, None; J.C. Folk, None; H.C. Boldt, None; K.M. Gehrs, None; R.F. Mullins, None; V.B. Mahajan, None.
  • Footnotes
    Support  Research to Prevent Blindness
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 4457. doi:
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      R. Somani, S. R. Russell, J. C. Folk, H. C. Boldt, K. M. Gehrs, R. F. Mullins, V. B. Mahajan; Immunohistochemical Study of Diamond-Dusted Tano Scratchers After Macular Surgery. Invest. Ophthalmol. Vis. Sci. 2009;50(13):4457.

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Abstract

Purpose: : To identify the tissues removed following application of Tano diamond-dusted scrapers to the macula.

Methods: : A Tano diamond-dusted scraper was applied to the macula of human donor eyes. The retina was fixed in 4% paraformaldehyde and processed for immunohistochemistry. Anti-laminin and DAPI stain were used to identify the internal limiting membrane and cell nuclei. A similar analysis was performed directly on the instrument tips following vitrectomy for macular hole.

Results: : The macula showed distinct areas of limited and complete ILM tissue disruption. The tips of the Tano diamond-dusted tip scraper showed laminin positive tissue fragments and limited numbers of cells.

Conclusions: : When applied to the macula, brushing the Tano diamond-dusted scraper disrupts the ILM of the retina and may remove surface cells. This technique may be sufficient to achieve macular hole closure at rates comparable to complete ILM peeling.

Keywords: macular holes • vitreoretinal surgery • microscopy: light/fluorescence/immunohistochemistry 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2015 Association for Research in Vision and Ophthalmology.
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