April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Dermcidin Expression in the Eye - Potential Protective Mechanism Against Ocular Bacterial Invasion
P. J. McDonnell, III; S. Martins; J. Castro-Combs; W. Camacho; P. Whittman; J. L. Hicks; A. M. De Marzo; C. G. Eberhart; A. Behrens
Author Affiliations & Notes
  • P. J. McDonnell, III
    Ophthalmology, Johns Hopkins Wilmer Eye Inst, Baltimore, Maryland
  • S. Martins
    Ophthalmology, Johns Hopkins Wilmer Eye Inst, Baltimore, Maryland
  • J. Castro-Combs
    Ophthalmology, Johns Hopkins Wilmer Eye Inst, Baltimore, Maryland
  • W. Camacho
    Ophthalmology, Johns Hopkins Wilmer Eye Inst, Baltimore, Maryland
  • P. Whittman
    Ophthalmology, Johns Hopkins Wilmer Eye Inst, Baltimore, Maryland
  • J. L. Hicks
    Pathology, Johns Hopkins, Baltimore, Maryland
  • A. M. De Marzo
    Pathology, Johns Hopkins, Baltimore, Maryland
  • C. G. Eberhart
    Ophthalmology, Johns Hopkins Wilmer Eye Inst, Baltimore, Maryland
  • A. Behrens
    Ophthalmology, Johns Hopkins Wilmer Eye Inst, Baltimore, Maryland
  • Footnotes
    Commercial Relationships  P.J. McDonnell, III, Developer/Patent, P; S. Martins, None; J. Castro-Combs, None; W. Camacho, None; P. Whittman, None; J.L. Hicks, None; A.M. De Marzo, None; C.G. Eberhart, None; A. Behrens, Patent, P.
  • Footnotes
    Support  Fight for Sight
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 5110. doi:
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      P. J. McDonnell, III, S. Martins, J. Castro-Combs, W. Camacho, P. Whittman, J. L. Hicks, A. M. De Marzo, C. G. Eberhart, A. Behrens; Dermcidin Expression in the Eye - Potential Protective Mechanism Against Ocular Bacterial Invasion. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5110.

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Abstract

Purpose: : Dermcidin (DCD), a recently discovered antimicrobial peptide, has been previously detected in the aqueous humor of New Zealand white rabbits. The purpose of this study is to detect the presence of DCD in human intraocular tissues as a potential mechanism of innate defense against intraocular infections.

Methods: : We investigated the immunoexpression of this novel and established antimicrobial peptide in the human eye using a mouse monoclonal antibody raised against human DCD chromatographically purified from human sweat. 5-µm vertical paraffin sections were prepared and immediately processed. The sections were incubated for 30 minutes with a 1:600 dilution of mouse monoclonal IgM raised against human DCD. Regional production of this peptide was detected in several intraocular structures.

Results: : DCD was constitutively expressed in human sweat glands of all skin biopsies. Immunoreactivity was also observed in human corneal epithelium, endothelium and ciliary body. Human corneal endothelium and ciliary body seem to express DCD gene constitutively.

Keywords: endophthalmitis • protective mechanisms • anterior chamber 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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