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H. Xu, Q. Jian, H. Xie, Z. Yin; Activation of Retinal Stem Cells in the Proliferating Marginal Region of RCS Rats During Development of Retinitis Pigmentosa. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5146.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the proliferation of retinal stem cells in the proliferating marginal region of RCS rats during development of Retinitis Pigmentosa.
RCS rats were divided into 3 groups randomly: the initial stage group (P15d), the mid-stage group (P30d) and the advanced stage group (P90d) according to the severity of degeneration (n=4 in each group). Age-matched Long-Evan’s rats without retinal degeneration served as controls. The thickness of outer nuclear layer (ONL) was detected using DAPI immunofluorescence staining; A transcription factor (Chx-10) expressed by retinal stem cell/ progenitors and thymidine analog BrdU incorporated in proliferating cells were detected using immunofluorescence staining, and positive cells were counted per vision in the proliferating marginal region of the retinal.
Compared with Long-Evan’s rats age-matched, the thickness of ONL of RCS rats decreased as aging from P15, almost disappeared in P90 RCS rats. There was no significant difference between the quantity of Chx-10 positive cells in P15 RCS rats and in P15 Long-Evan’s rats(P>0.05). As compared with Long-Evan’s rats age-matched, the quantity of Chx-10 positive cells both in P30 and P90 RCS rats increased (P<0.05); While groups of RCS rats compared with each other, the P30 group increaced markedly (P<0.01). 3) There were more BrdU positve cells in goups of RCS rats than that in the groups of Long-Evan’s rats age-matched, in which BrdU positve cells could hardly be detected; Compared with P15 and P90 RCS rats, the positive cells in P30 RCS rats increaced markedly (P<0.01).
This indicated that retinal stem cells could be activated to proliferate at proliferating marginal region during a short time while retinal of RCS rats at P30 degenerated significantly.
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