April 2009
Volume 50, Issue 13
ARVO Annual Meeting Abstract  |   April 2009
Near-Infrared Light Confocal Imaging of Retinal Activity
Author Affiliations & Notes
  • Y. Li
    Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama
  • X. Yao
    Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama
  • Footnotes
    Commercial Relationships  Y. Li, None; X. Yao, None.
  • Footnotes
    Support  Eyesight Foundation of Alabama
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 5170. doi:
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    • Get Citation

      Y. Li, X. Yao; Near-Infrared Light Confocal Imaging of Retinal Activity. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5170.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To validate near infrared (NIR) reflected light imaging of fast intrinsic optical signals (IOSs) that have time courses comparable to ERG responses.

Methods: : A fast confocal imager was constructed to demonstrate reflected light detection of transient IOSs in isolated frog (Rana Pipiens) retinas. While an acousto-optic deflector (AOD) was employed to produce rapid, vibration-free scanning of the near infrared (NIR) illumination light, an ultrafast (68,000 lines/s) line-scan camera was used for parallel recording of multiple (1024 pixels/line) retinal points. During the experiment, a while light flash was used for retinal stimulation. By differentiating the pre-stimulus and after-stimulus images, dynamic IOS images were constructed to characterize spatial and temporal properties of stimulus-evoked neural activity in the retina.

Results: : Using the home-built confocal imager, NIR light imaging of fast retinal neural activity was demonstrated. Fast (>50 KHz) NIR light imaging disclosed transient reflected light changes tightly correlated with the stimulus-evoked retinal activity. High resolution (~um) images revealed both positive (increasing) and negative (decreasing) IOSs in adjacent retinal areas. Depth-resolved confocal imaging suggested the existence of multiple sources of transient IOSs, and different retinal neurons might contribute to the IOS image with variable time courses and polarities.

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • retina • electrophysiology: non-clinical 

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