April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Alpha-Crystallins Regulate DNA Damage Pathway to Prevent Stress-Induced Apoptosis of Human Lens Epithelial Cells
J. Liu; L. Gong; Z. Cao; M. Deng; D. Yuan; Q. Yan; H.-G. Chen; D. W. Li
Author Affiliations & Notes
  • J. Liu
    Bichemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • L. Gong
    Bichemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • Z. Cao
    Bichemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • M. Deng
    Bichemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • D. Yuan
    Bichemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • Q. Yan
    Bichemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • H.-G. Chen
    Bichemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
  • D. W. Li
    Bichemistry & Molecular Biology, University of Nebraska Med Center, Omaha, Nebraska
    Ophthalmology & Visual Sciences, University of Nebraska Medical Center, Omaha, Nebraska
  • Footnotes
    Commercial Relationships  J. Liu, None; L. Gong, None; Z. Cao, None; M. Deng, None; D. Yuan, None; Q. Yan, None; H.-G. Chen, None; D.W. Li, None.
  • Footnotes
    Support  1R01EY015765, UNMC start-up funds
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 5180. doi:
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      J. Liu, L. Gong, Z. Cao, M. Deng, D. Yuan, Q. Yan, H.-G. Chen, D. W. Li; Alpha-Crystallins Regulate DNA Damage Pathway to Prevent Stress-Induced Apoptosis of Human Lens Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5180.

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Abstract

Purpose: : In our previous studies, we have demonstrated that alpha-crystallins can negatively regulate stress-induced apoptosis through suppression of the ERK-mediated pathway and activation of the AKT signaling pathway. In the present study, we present evidence to show that alpha-crystallins can regulate the ATR-p53 pathway to prevent UVA-induced apoptosis.

Methods: : UVA was used to irradiate human lens epithelial cells stably expressing vector, alphaA, and alphaB. Western blot analysis was used for detection of ATR, CHK1/2 and p53 activation. Reporter gene activity assay was used to explore the transactivity of p53. Hoechst staining was used for apoptosis assay.

Results: : Human lens epithelial cells expressing either alphaA- or alphaB-crystallin are substantially resistant to UVA-induced apoptosis. UVA-induces activation of ATR and CHK1/2 kinases to activate p53 in vector-transfected cells. However, in alphaA- or alphaB-crystallin-transfected cells, activation of ATR, CHK1/2 kinases and p53 was distinctly suppressed.

Conclusions: : Alpha-crystallins suppress the DNA damage pathway to prevent UVA-induced apoptosis.

Keywords: crystallins • cell survival • cataract 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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