Purchase this article with an account.
Y. Zhang, M. Mowrey-Mckee, M. M. Gabriel, L. D. Hazlett; Contact Lens Wear and Expression of Inflammatory Cytokines After LPS Challenge. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5635.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Lipopolysaccharide (LPS) may be a contaminant of either devices used in the eye, or a contaminant of topical ophthalmic solutions. The purpose of the study was to test expression of corneal inflammatory cytokines after topical LPS challenge in mice (corneas intact or scarified) and compare these data with contact lens wearing rats exposed to similar LPS concentrations.
For mice, the left central corneal epithelium of C57BL/6 and BALB/c mice was either scarified (X3 one mm incisions) or was left intact, and 5 µl of LPS (0.02ng/µl, 20ng/µl and 2µg/µl from Pseudomonas aeruginosa, serotype 10) or endotoxin free water (EFW) was topically applied. Lewis rats wore a lotrafilcon B contact lens in the left eye for 2 weeks. After, lenses were removed and rats were challenged by placing a EFW-or LPS-soaked contact lens (concentrations of LPS as above) on the same eye. After 24 h, real time RT-PCR was used to evaluate the corneal response.
In the scarified cornea of mice challenged topically with LPS at a concentration of 2µg/µl (50,000 Endotoxin units, EU), mRNA levels of IL-1β, IL-6, MIP-2, TGF-β, TNF-, TLR-4 and MyD88 were all significantly upregulated compared with EFW controls. In the 20ng/µl (500 EU) group, only IL-1β, TLR-4 and MyD88 were upregulated over controls. In the 0.02ng/µl (0.5 EU) group, none of the above cytokines were upregulated. In contrast, in mice with an intact cornea, none of the above cytokines was upregulated at any LPS concentration tested. In contact lens wearing rats, only TNF- and CINC-1 were significantly upregulated in all LPS treated groups compared with EFW controls. Levels of IL-1β, IL-6, TGF-β1, TLR-4 and MyD88 were not different from controls.
These data provide evidence that an intact cornea can tolerate higher concentrations of LPS without significant upregulation of cytokines when compared with wounding or with contact lens wear.
This PDF is available to Subscribers Only