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I. Grulkowski, M. Gora, M. Szkulmowski, I. Gorczynska, D. Szlag, S. Marcos, A. Kowalczyk, M. Wojtkowski; High Speed in vivo Anterior Segment Imaging by Spectral Optical Coherence Tomography. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5797.
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© ARVO (1962-2015); The Authors (2016-present)
To present the performance of high speed spectral OCT (sOCT) system for in vivo imaging of the entire anterior segment of eye.
A new sOCT system with a CMOS camera as a spectrometer detector was developed. The design allows achieving axial measurement ranging up to 10 mm, with a speed of 135,000 A-scans/second. Axial and transverse resolutions in tissue are 6.9 um and 27 um, respectively. Measurements were performed on four healthy volunteers. The high speed provided by CMOS camera enabled reconstruction of three dimensional structure of the anterior segment, with arbitrary density of sampling points in all three directions. We developed optimized two- and three-dimensional scanning protocols containing of consecutive B-scans or 3D data sets, which increases the flexibility of the system and allows implementation of new imaging protocols.
We obtained high-resolution two- and three-dimensional OCT tomograms of cornea, lens, iris and anterior chamber angle in normal subjects. The high acquisition rates and optimized scanning protocols allowed measurement of the anterior segment dynamics during lenticular accommodation, blinking and pupillary reaction to light stimulus. This is the first time, to our knowledge, that three-dimensional high quality images of the entire anterior segment of the eye from the cornea up to posterior part of the lens capsule, and two-dimensional cross-sections are obtained in real-time.
High-speed OCT imaging of the anterior segment is demonstrated at up to 135,000 A-scans/second using spectral OCT system. High acquisition speed enables rapid acquisition of three-dimensional data for the studies of biomechanics of anterior segment, and for the study of dynamic accommodation. Optimized scanning protocols can be used for efficient static and dynamic imaging of anterior segment structures. The system has potential to become a powerful tool for quantitative analysis of anterior segment of eye and its dynamics.
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