April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Using Hyperspectral Imaging to Investigate Oxygen Saturation in ONH Tissue in Primate’s Eyes during the Development of Experimental Early Phase Glaucoma
B. Khoobehi; H. Wafapoor; A. M. Eaton; M. Moghareh; R. Grigorian; S. Miles; E. Puissegur; E. Tinsley
Author Affiliations & Notes
  • B. Khoobehi
    Department of Ophthalmology, LSU Eye Center, New Orleans, Louisiana
  • H. Wafapoor
    Department of Ophthalmology, Private Practice, Fort Myers, Florida
  • A. M. Eaton
    Department of Ophthalmology, Private Practice, Fort Myers, Florida
  • M. Moghareh
    Department of Ophthalmology, LSU Eye Center, New Orleans, Louisiana
  • R. Grigorian
    Department of Ophthalmology, LSU Eye Center, New Orleans, Louisiana
  • S. Miles
    Department of Ophthalmology, LSU Eye Center, New Orleans, Louisiana
  • E. Puissegur
    Department of Ophthalmology, LSU Eye Center, New Orleans, Louisiana
  • E. Tinsley
    Department of Ophthalmology, LSU Eye Center, New Orleans, Louisiana
  • Footnotes
    Commercial Relationships  B. Khoobehi, None; H. Wafapoor, None; A.M. Eaton, None; M. Moghareh, None; R. Grigorian, None; S. Miles, None; E. Puissegur, None; E. Tinsley, None.
  • Footnotes
    Support  NIH grants EY012887 and EY02377 and by an unrestricted grant from Research to Prevent Blindness, NY, NY
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 5804. doi:
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      B. Khoobehi, H. Wafapoor, A. M. Eaton, M. Moghareh, R. Grigorian, S. Miles, E. Puissegur, E. Tinsley; Using Hyperspectral Imaging to Investigate Oxygen Saturation in ONH Tissue in Primate’s Eyes during the Development of Experimental Early Phase Glaucoma. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5804.

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Abstract

Purpose: : To investigate oxygen saturation in ONH tissues and in retinal artery/vein pairs in experimentally induced early- phase glaucoma using a primate animal model.

Methods: : Hyperspectral imaging (HSI) that interfaces with a fundus camera was used to detect the spectral signature of oxy- and deoxyhemoglobin from reflected light. Captured images were broken down to 100 different images from 500-700 nm with 2 nm resolution. Five normal cynomolgus monkeys 4 to 4.5 years of age and 2.5 to 3 kg body weight were used. For an HSI session (oxygen saturation measurement), each monkey was anesthetized. HIS imaging was performed every other week for a period of 10 months. One eye of each monkey was lasered (trabecular meshwork). The laser power was 300-1500 mW, duration 100 ms and the spot size was 50 µm. The laser procedure was repeated until the intraocular pressure (IOP) maintained a stable and moderate elevation. Compliance testing involving the use of confocal scanning laser tomography to measure the parameter mean position of the disc (MPD), which describes the position of the surface of the ONH relative to the peripapillary retinal nerve fiber layer. Both eyes of each monkey were compliance tested on three separate occasions to provide the pre-laser MPD ± 95% confidence interval for normal eyes.

Results: : The IOP of a normal eye is 7.8 mm Hg and the IOP of a lasered eye is 12.82 mm Hg. On the average, 8 weeks were required to achieve early glaucoma (EG) from onset of IOP elevation. The results of oxygen saturation calculated from oxy/deoxy spectrum are summarized in the Table.

Conclusions: : The differences between the various tissues including the vein were significantly different before and after the onset of glaucoma, except for the artery. This technique detected changes in oxygen saturation as low as 4%

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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