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S. Johnsen-Soriano, M. Sancho-Tello, E. Arnal, A. Navea, M. Diaz-Llopis, F. Bosch-Morell, F. Romero; Retinal Inflammatory Markers in Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5893.
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To study T-helper cell cytokine profiles of naive chemokine and cytokine in retinal tissue of diabetic subjects.
Cytokines (interleukin IL-1a, IL-1b, IL-2, IL-4, IL-6, IL-10, TNFa, GM-CSF, interferon [IFN]-gamma) and Chemokines (MIP-1a, MIP-2, MIP-3a, MCP-1, GRO/KC, RANTES, Fractalkine), in addition to tyrosine nitration were measured in retinal homogenate obtained from Long Evans rats with five months experimental diabetes.
The Th1 cytokines; Interleukin-2 and INF-g, in addition to NO production were found to be significantly elevated in diabetic rat retina. None of the other cytokines and nor chemokines studied were affected by the diabetic condition.
Immunoregulatory cytokines belonging to the TH-1 group (IL-2 and IFN-g) were detected in retinal homogenate from rats with experimental diabetes, indicating a T-helper type 1 response and suggesting novel roles for IL-2 and INF-gamma. Moreover, the NO production was significantly elevated in the diabetic retina, supporting the concept of an inflammatory element in the development of diabetic retinopathy.
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