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E. C. LaHey, L. J. A. G. Ricker, S. C. Dieudonne, E. S. Rennel, T. T. J. M. Berendschot, A. Kijlstra, F. Hendrikse; Anti-Angiogenic Isoforms of Vegf Predominate in Subretinal Fluid of Patients With Rhegmatogenous Retinal Detachment and Proliferative Vitreoretinopathy. Invest. Ophthalmol. Vis. Sci. 2009;50(13):6089.
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Vascular endothelial growth factor (VEGF) plays an essential role in the development of physiological and pathological angiogenesis. In proliferative vitreoretinopathy (PVR), a non-angiogenic eye disease that is characterized by the formation of mainly avascular membranes, VEGF levels are found to be up-regulated. Recently, it was discovered that VEGF is alternatively spliced to form the angiogenic (VEGFxxx) and anti-angiogenic (VEGFxxxb) family of isoforms. Previous studies on expression of VEGF in PVR samples have not distinguished between the two families of isoforms.
Total VEGF and VEGFxxxb levels were measured in subretinal fluid of patients with PVR (n = 10) and uncomplicated rhegmatogenous retinal detachment (RRD; n = 27) using enzyme-linked immunosorbent assay
Total VEGF levels in PVR patients were significantly higher than in patients with uncomplicated RRD (p = 0.047). Anti-angiogenic VEGFxxxb isoforms predominate and account for about two-thirds of the total VEGF in both groups: 69% in the PVR negative samples compared to 64% in the PVR positive samples.
The absence of an angiogenic switch of VEGF may explain a lack of blood vessels in PVR membranes. Elevated VEGF levels indicate that this cytokine may play a role in the pathogenesis of PVR that is not related to angiogenesis.
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