April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Perturbed Intraepithelial Differentiation and Accelerated Healing in Corneal Epithelium of Epiplakin-Null Mice
Author Affiliations & Notes
  • M. Kokado
    Department of Ophthalmology,
    Wakayama Medical University, Wakayama, Japan
  • Y. Okada
    Department of Ophthalmology,
    Wakayama Medical University, Wakayama, Japan
  • K. Ishikawa
    Department of Anatomy, Biology and Medicine, Faculty of Medicine, Oita University, Hasama-machi, Japan
  • M. Goto
    Department of Anatomy, Biology and Medicine, Faculty of Medicine, Oita University, Hasama-machi, Japan
  • S. Fujiwara
    Department of Anatomy, Biology and Medicine, Faculty of Medicine, Oita University, Hasama-machi, Japan
  • M. Miyajima
    Laboratory Animal Center,
    Wakayama Medical University, Wakayama, Japan
  • S. Saika
    Department of Ophthalmology,
    Wakayama Medical University, Wakayama, Japan
  • Footnotes
    Commercial Relationships  M. Kokado, None; Y. Okada, None; K. Ishikawa, None; M. Goto, None; S. Fujiwara, None; M. Miyajima, None; S. Saika, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 6283. doi:
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      M. Kokado, Y. Okada, K. Ishikawa, M. Goto, S. Fujiwara, M. Miyajima, S. Saika; Perturbed Intraepithelial Differentiation and Accelerated Healing in Corneal Epithelium of Epiplakin-Null Mice. Invest. Ophthalmol. Vis. Sci. 2009;50(13):6283.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To examine the roles of Epiplakin (EPPK) in the intraepithelial differentiation and healing of a defect in corneal epithelium by using ERRK-deficient mice.

Methods: : (1) Ten EPPK-/- (KO) and 10 EPPK+/+ (WT) mice were used. To evaluate the intraepithelial differentiation from basal cells to superficial cells, histology and ultrastructure of the corneal epithelium was observed. The expression of keratin 14 (K14), a specific keratin of basal cells of stratified epithelia, was examined to analyze intraepithelial epithelial differentiation. (2) A circular defect was created in central cornea of the right eye of 10 KO and 10 WT mice by using a 2-mm trephine and a blade. The closure of the epithelial defect was determined by fluorescein staining.

Results: : (1) Morphologically basal cell-like epithelial cells were observed in the supra-basal layer in corneal epithelium of KO mice, where more differentiated cells were seen in WT corneal epithelium. Such suprabasal cells of an abnormal morphology were labeled with anti-K14 antibody in KO corneal epithelium, while K14-positive cells were observed in basal layer in corneal epithelium of WT mice (2) Loss of EPPK accelerated the wound closure in corneal epithelium form 18 to 24 hrs post-wounding (p<0.05). K-14 positive cells were observed in migrating corneal epithelium of both WT and KO mice.

Conclusions: : The absence of EPPK disturbs the differentiation from basal cells to superficial cells in corneal epithelium in mice. Lacking EPPK accelerates migration of healing corneal epithelium. Abundance of K14-labeled basal cell-like cells in suprabasal layer might account for the promotion of epithelial healing.

Keywords: wound healing • cornea: epithelium • cell adhesions/cell junctions 
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