April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Impact of Hyaluronic Acid, Panthenol and Its Combination on Epithelial Wound Healing in Murine Corneas
Author Affiliations & Notes
  • F. Bucher
    Ophthalmology, University of Erlangen-Nuernberg, Erlangen, Germany
  • B. Bachmann
    Ophthalmology, University of Erlangen-Nuernberg, Erlangen, Germany
  • F. Bock
    Ophthalmology, University of Erlangen-Nuernberg, Erlangen, Germany
  • D. Gross
    Ursapharm Arzneimittel GmbH & Co. KG, Saarbrücken, Germany
  • C. Cursiefen
    Ophthalmology, University of Erlangen-Nuernberg, Erlangen, Germany
  • F. E. Kruse
    Ophthalmology, University of Erlangen-Nuernberg, Erlangen, Germany
  • Footnotes
    Commercial Relationships  F. Bucher, funding of materials and services, F; B. Bachmann, None; F. Bock, None; D. Gross, employee of Ursapharm, E; C. Cursiefen, None; F.E. Kruse, funding of materials, F.
  • Footnotes
    Support  German research foundation SFB643
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 6285. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      F. Bucher, B. Bachmann, F. Bock, D. Gross, C. Cursiefen, F. E. Kruse; Impact of Hyaluronic Acid, Panthenol and Its Combination on Epithelial Wound Healing in Murine Corneas. Invest. Ophthalmol. Vis. Sci. 2009;50(13):6285. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Currently there are no approved drugs which enhance corneal epithelial wound healing. Hyaluronic acid and Panthenol are approved for the therapy of dry eye. Hyaluronic acid is a ligand for the cell surface adhesion molecule CD44 and by this enhances the migration of corneal epithelial cells. In organisms Panthenol becomes quickly oxidized to pantothenic acid (vitamin B5) which is used in the synthesis of coenzyme A and thus is crucial for metabolic functions. Aim of this study was to test the effect of hyaluronic acid, Panthenol and its combination in a mouse model of corneal erosion.

Methods: : C57BL/6 mice (age: 6-8 weeks, n=16 per group) were used in all experiments. The central corneal epithelium was demarcated with a 2 mm trephine and subsequently removed using a blade. Corneas were stained with fluorescein. Pictures were taken under blue light directly after removal of the epithelium and 24 hrs thereafter. 0,1% hyaluronic acid, 2% Panthenol and the combination of 0,1% hyaluronic acid and 2% Panthenol (Hylo-Care®) were tested (eye drops, 5µl, 12x/day). 0.9% NaCl was used as negative control. Pictures were analysed by using the software Cell^F (Olympus, Hamburg, Germany).

Results: : The re-epithelization was accelerated when compared to 0.9% NaCl in all corneas. The remaining site of the epithelial defect after 24 hrs was 12.68% + 9.53 (p<0.001) for Hylo-Care®, 14.77% + 13.72 (p<0.001) for hyaluronic acid and 23.92% + 23.94 (p<0.012) for Panthenol.

Conclusions: : Both hyaluronic acid and Panthenol are effective for the therapy of corneal epithelial defects. This effect seems to be additive in the combination of the active components.

Keywords: cornea: epithelium • wound healing • cornea: tears/tear film/dry eye 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×