April 2009
Volume 50, Issue 13
ARVO Annual Meeting Abstract  |   April 2009
High-Fat Diet Delays Mouse Corneal Epithelial Wound Healing
Author Affiliations & Notes
  • Z. Li
    Leukocyte Biology,
    Baylor College of Medicine, Houston, Texas
    Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, China
  • D. Brake
    Leukocyte Biology,
    Baylor College of Medicine, Houston, Texas
  • C. W. Smith
    Leukocyte Biology,
    Children Nutrition Research Center,
    Baylor College of Medicine, Houston, Texas
  • Footnotes
    Commercial Relationships  Z. Li, None; D. Brake, None; C.W. Smith, None.
  • Footnotes
    Support  NIH grant EY018239-01A1, EY017120-02; USDA grant 6250-51000-046-01A; China NNFS grant 39970250 and 30672287
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 6287. doi:
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    • Get Citation

      Z. Li, D. Brake, C. W. Smith; High-Fat Diet Delays Mouse Corneal Epithelial Wound Healing. Invest. Ophthalmol. Vis. Sci. 2009;50(13):6287.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : This study tested the hypothesis that a high-fat diet impairs corneal wound healing and inflammation.

Methods: : Female C57BL/6H mice were fed either a standard chow diet or a diet high in saturated fatty acids (21% by weight milk fat). After 6 weeks, a corneal wound was made by removal of a 2-mm diameter area of central corneal epithelium. Re-epithelialization, epithelial proliferation, γΔ T cell and neutrophil emigration were evaluated at different time points after wounding.

Results: : Though mice on the high fat diet were not significantly obese, γΔ T cell density in the epithelial cell layer of ocular surface, including conjunctiva and corneal limbus, was significantly higher (>3 times, p<0.001) than that of mice on standard chow diet. In addition, animals on the high fat diet presented delayed wound re-epithelialization (20% reduction, p<0.05), decreased epithelial cell proliferation (~50% reduction, p<0.01), and blunted neutrophil infiltration (~40% reduction p<0.01). While γΔ T cells increase significantly in lean mice following corneal abrasion, their number did not significantly change after corneal abrasion in the mice on the high fat diet.

Conclusions: : These findings indicate that a short-term high-fat diet changed the distribution of γΔ T cells on the ocular surface and exerts negative effects on corneal epithelial wound healing, with dysregulation of the inflammatory processes.

Keywords: cornea: epithelium • wound healing • inflammation 

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