April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
ICAM-1 Is Necessary for Efficient Corneal Wound Healing and Recruitment of Gamma Delta T Cells
Author Affiliations & Notes
  • S. Byeseda
    Section of Leukocyte Biology, Department of Pediatrics, Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas
  • A. R. Burns
    Section of Leukocyte Biology, Department of Pediatrics, Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas
    College of Optometry, University of Houston, Houston, Texas
  • C. W. Smith
    Section of Leukocyte Biology, Department of Pediatrics, Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas
  • Z. Li
    Section of Leukocyte Biology, Department of Pediatrics, Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas
    Laboratory for Regenerative Medicine, Jinan University, Guangzhou, China
  • Footnotes
    Commercial Relationships  S. Byeseda, None; A.R. Burns, None; C.W. Smith, None; Z. Li, None.
  • Footnotes
    Support  NIH Grant EY018239-01A1, EY017120-02; USDA Grant 6250-51000-046-01A; China NNFS Grant 39970250, 30672287
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 6294. doi:
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      S. Byeseda, A. R. Burns, C. W. Smith, Z. Li; ICAM-1 Is Necessary for Efficient Corneal Wound Healing and Recruitment of Gamma Delta T Cells. Invest. Ophthalmol. Vis. Sci. 2009;50(13):6294.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We found that γΔ T cells migrate into corneal epithelium in response to epithelial wounding, and healing and inflammation are significantly reduced in TCRΔ-/- mice (Am. J. Pathol 171:838-845, 2007). The current work is to define the contribution of Intercellular Adhesion Molecule-1 (ICAM-1) to corneal wound healing and γΔ T cell migration into the epithelium.

Methods: : Limbal and paralimbal corneal epithelium was analyzed by qrtPCR, electron microscopy, and immunocytology at various time points after central corneal epithelial abrasion in female wildtype, ICAM-1-/- and P-selectin-/- C57BL/6 mice.

Results: : Epithelial ICAM-1 mRNA was low at baseline, increased 26 fold at 3 hrs after abrasion, and returned to basal levels at 18 hrs. Re-epithelialization and epithelial cell division were significantly reduced (~50% at 18 hrs, p<0.01) after abrasion in ICAM-1-/- mice versus wild type, and at 96 hrs, recovery of epithelial thickness was only 66% (p<0.01) of wild type. ICAM-1-/- mice had 50.9% (p<0.01) fewer γΔ T cells resident in the limbus at baseline and failed to show significant changes in their number following abrasion. In contrast, wildtype mice had a 40% reduction of these cells at 3 hrs after abrasion followed by a ~5 fold increase at 24 hrs. Anti-ICAM-1 blocking antibody in wild type mice reduced the number of epithelial γΔ T cells to a number comparable to ICAM-1-/- mice. P-selectin-/- mice exhibited normal γΔ T cell kinetics in the epithelium following abrasion, and control antibodies failed to alter normal kinetics.

Keywords: cornea: epithelium • wound healing • inflammation 
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